NEST Scientific 726001 100mm Cell Culture Insert-Dish 3.0um PC Membrane Translucent TC Treated Sterile

Transparent PET membrane provides clear visibility for observation. Create a cell culture environment that closely mimics in vivo conditions. Inserts allow polarized cells to perform metabolic functions more naturally by allowing nutrient uptake from both the apical and basolateral sides. Grow cells in co-culture systems either with direct cell-to-cell interaction or separated without physical contact. Treated to promote maximum cell adhesion. Membrane requires staining to be visible. The membrane features a low adsorption rate, minimizing the loss of small molecular proteins and other compounds. Transparent PET inserts provide high optical clarity, enabling clear visualization of cell outlines under phase-contrast microscopy.

Features:

• Plasma Etched Cell Culture Dish 
• Insert have translucent, 3.0 um pore, PET membrane
• Individually Wrapped 
• Insert Diameter - 75mm
• Dish volume - 13mL
• Inner volume of insert - 9mL
• Growth area of insert membrane - 44 cm²
• Nominal pore density 2x10^6 

Quality Details:

• USP grade VI virgin polystyrene
• USP grade VI virgin PET
• Manufactured in cGMP (21 CFR part 820/821) compliant factory
• Produced in ISO 13485:2016 and ISO 9001 facility
• Molded and packed in ISO 7 cleanroom
• E-beam sterilized and lot tested to SAL 10^-6
• Lot tested free of pyrogens USP 85
• Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
• Lot tested free DNase/RNase and DNA 
• Lot tested free BSE/TSE
• Certified free of animal products

Cell and tissue culture technologies are increasingly essential in both basic research and applied life sciences. To better replicate in vivo conditions, advanced culture vessels and specialized surface treatments for cell adhesion are continually being developed, particularly for the growth of sensitive or specialized cell lines.

Among these innovations, permeable supports with microporous membranes have become a key tool. By allowing molecular exchange at both the apical and basal surfaces, they promote the growth of polarized cells and support secretion and absorption in a way that closely mirrors natural metabolic processes. This creates a more physiologically relevant environment, making permeable supports an effective solution for cultivating complex cell types.