NEST Scientific 725111 12 Cell Culture Inserts+24 Well Plate 0.4um PC Membrane Opaque Non-Treated Sterile

PC membrane inserts are ideal for tumor cell migration and invasion studies, hematostaxis or endothelial cell migration assays and co-culture applications. Create a cell culture environment that closely mimics in vivo conditions. Inserts allow polarized cells to perform metabolic functions more naturally by allowing nutrient uptake from both the apical and basolateral sides. Grow cells in co-culture systems either with direct cell-to-cell interaction or separated without physical contact. Membrane requires staining to be visible. The membrane features a low adsorption rate, minimizing the loss of small molecular proteins and other compounds. PC is compatible with common solvents used for fixation and staining.

Features:

• 24 Well Non-Treated - Culture Plates (702011) 
• Inserts have opaque, 0.4 um pore, polycarbonate membrane
• 12 Inserts included with each plate
• 12 plates per pack, 48 plates total per case
• Individually Wrapped - 1 per blister pack - 48 plates and 576 inserts per case
• Insert Diameter - 6.5mm
• Plate volume per well - 0.6mL
• Inner volume of insert - 0.1mL
• Growth area of insert membrane - 0.33 cm²
• Nominal pore density 1x10⁸ pores per cm²
• Used for primary cells, tumor, stem cells

Quality Details:

• USP grade VI virgin polystyrene
• USP grade VI virgin polycarbonate
• Manufactured in cGMP (21 CFR part 820/821) compliant factory
• Produced in ISO 13485:2016 and ISO 9001 facility
• Molded and packed in ISO 7 cleanroom
• E-beam sterilized and lot tested to SAL 10^-6
• Lot tested free of pyrogens USP 85
• Lot tested free of cytotoxins/pyrogens In Vivo USP 88 and In Vitro USP 87
• Lot tested free DNase/RNase and DNA 
• Lot tested free BSE/TSE
• Certified free of animal products

Cell and tissue culture technologies are increasingly essential in both basic research and applied life sciences. To better replicate in vivo conditions, advanced culture vessels and specialized surface treatments for cell adhesion are continually being developed, particularly for the growth of sensitive or specialized cell lines.

Among these innovations, permeable supports with microporous membranes have become a key tool. By allowing molecular exchange at both the apical and basal surfaces, they promote the growth of polarized cells and support secretion and absorption in a way that closely mirrors natural metabolic processes. This creates a more physiologically relevant environment, making permeable supports an effective solution for cultivating complex cell types.