IBI Scientific SS-2002S TUNAIR Half-Baffle Polypropylene Erlenmeyer Flask Kit 300 mL w/ Silicone Filters

The TUNAIR™ polypropylene Erlenmeyer Flask Systems are a unique and patented flask and closure system, designed for microbiology and biotechnology applications. This system provides optimum growth conditions for aerobic microorganisms, mammalian cells, and plant cells. They also provide better culture growth and productivity than standard Erlenmeyer flasks.

The kit includes:

• Comes complete with twelve (12) polypropylene 300 ml Half-Baffle TUNAIR™ Polypropylene Erlenmeyer Flasks, twelve (12) two-piece caps, and fifteen (15) silicone filters.

Additional Item Description:

The TUNAIR™'s high oxygen absorption rate is due to the unique baffling and turbo-vane closure design. The TUNAIR™ flask systems are designed to increase the availability of dissolved oxygen as well as improve cell yields.

The Half-Baffle Shake Flask has two baffles that produce a vortex motion. The working volume is 100 ml.

The two piece cap assemblies are constructed of polypropylene and are resistant to most solvents. All flasks and caps are fully autoclavable prior to reuse and filter linings can also be autoclaved or simply replaced.

Replacement Instructions:

To replace the filter lining in the cap assembly, simply pinch the flanges of the inner-closure shell until they snap loose. Then, pull apart and remove used lining. Replace the lining by sandwiching it between two parts of the cap and snap the cap back together. When reassembling the cap, ensure the flanges from the inner piece snap into the mated grooves in the outer piece. This will ensure the cap assembly stays together during use.

All TUNAIR™ flasks and caps can be cleaned by soaking in water with a light detergent solution to loosen dirt and contaminants, then air dry.

The flasks are constructed of polypropylene and resistant to most solvents.

All TUNAIR™ flasks can be cleaned by soaking in water with a light detergent solution to loosen dirt and contaminants, then air dry.

300 ml flask:

Allows for approx. 150 ml (max) of working volume for culturing cells

Half-Baffle:

The bottom inside of the flask has 2 raised baffles. This configuration will generate a single vortex within the media inside the flask when fastened to an orbital shaker. The half-baffle configuration will allow for slower operational rpm of the shaker while maintaining the single vortex. Less splashing occurs with the half-baffle flask which results in less cell death and higher cell densities.

Two Piece Cap:

The 300 ml flask and 2.5 L flask both have a special cap and filter system that promotes gas exchange and maintains sterility of the growth culture during use. The inner portion of the cap unsnaps from the outer portion by pushing in the tabs on the side of the cap assembly and then pulling the insert out.

0.2-micron filters: 

Silicone - This is a silicone filter that will last the life of the flask. This filter can be autoclaved while in the cap and on the flask for many times.

Cap & Filter assembly: 

Once you have the 2-piece cap assembly apart place the corresponding filter size inside the cap and the re-assemble the inner and outer cap pieces making certain to align the tabs with their associated holes. Once clipped into place, make certain the filter completely covers the cap assembly openings. 

Attaching the flasks to your shaker: 

IBI TUNAIR flasks can be used with nearly all standard shakers and shaker clamps. The 300 ml flask typically fits best in the 250 ml shaker clamp.

Preparing and Cleaning the TUNAIR flask: 

Your TUNAIR flasks are constructed of polypropylene plastic which allows them to be autoclaved. Once you have prepared your media inside the flask you can place the cap & filter on the flask and insert into the autoclave. Set the autoclave for wet cycle.

Once you have harvested your cells, wash the TUNAIR flask in a mild dish soap such as Dove Dish Soap then rinse well with DI water. The silicone filter can also be rinsed with DI water if needed. Observe the condition of the filters between runs to make certain there are no holes or tears in the filter. If there are, replace the filter immediately.