IBI Scientific RPM-201 Recombinant Mouse Protein FGF Basic 16.2 kDa 25 ug

Recombinant Mouse FGF Basic Protein (16.2 kDa)

IBI's recombinant mouse FGF basic protein offers exceptional quality for research focused on cell growth, development, and repair. Produced using a Pichia pastoris (yeast) system, this protein undergoes precise folding and post-translational modifications, delivering greater bioactivity compared to E. coli-derived alternatives. Free from endotoxins, HIS-TAGS, and carriers, this protein closely mirrors its native form and has a predicted molecular weight of 16.2 kDa, making it ideal for a variety of experimental applications.

Key Role of FGF Basic in Biological Processes

Fibroblast Growth Factors (FGFs) are critical regulators in processes such as angiogenesis, wound healing, and embryonic development. As members of the FGF family, FGF basic (FGF2) and FGF acidic (FGF1) are potent multipotential growth factors that support cell proliferation, migration, and differentiation. FGF family members interact with heparan sulfate proteoglycans on cell surfaces to activate essential signaling pathways, further emphasizing their role in tissue repair and development.

IBI's recombinant mouse FGF basic protein is lyophilized and shipped at room temperature without carrier protein. It can be reconstituted in sterile PBS with a carrier such as BSA or cell assay media. Suitable for use in cell culture, ELISA standards, and Western Blot controls, this protein is an indispensable tool for advancing research in cell biology and regenerative medicine.

Technical Information:

What is an acceptable carrier protein for recombinant protein reconstitution?

A carrier protein is an additional protein added to the lyophilized recombinant protein during reconstitution to increase the stability of the diluted recombinant protein during storage, freezing, and use.

Bovine Serum Albumin (BSA) is the most common carrier protein used for recombinant protein reconstitution. However, if working with bovine recombinant protein, use a non-bovine carrier protein such as Equine Serum Albumin (ESA). 

If using the recombinant protein for in vitro cell culture stimulation, it is often useful to reconstitute the recombinant protein directly in the complete cell culture medium containing serum or other acceptable carrier protein instead of a BSA/ESA solution.

How do I reconstitute the recombinant protein?

Most of the recombinant proteins can be reconstituted in sterile phosphate-buffered saline with 0.1% carrier protein, which is most commonly Bovine Serum Albumin (BSA).

If protein vial is left unopened, it can be stored at room temperature or -20°C for up to 12 months. 

Once protein is open and reconstituted, it must be stored at -20°C and is viable for up to 3 months. 

Avoid repeated freeze/thaw cycles as this could cause denaturation of proteins.

Typically, 100 µL is an acceptable reconstitution volume for 5 µg and 25 µg vials of recombinant protein. IBI's 5 µg and 25 µg vials have a maximum volume of 500 µL.

Typically, 1 mL is an acceptable reconstitution volume for 100 µg vials of recombinant protein. IBI's 100 µg vials have a maximum volume of 2 mL

What concentration of carrier protein is required for reconstitution?

The concentration of carrier protein should be at least 0.1% in the final solution. Additional carrier protein can be used, if desired. Typically, a 0.1-10% BSA solution in 1X PBS is an acceptable reconstitution solution.

What concentration is the recombinant protein upon reconstitution?

The concentration of recombinant protein is dependent on the reconstitution volume. For example, if reconstituting 5 µg of recombinant protein in 100 µL carrier protein solution, the concentration is 5 µg/100 µL or 50 µg/mL

What is the activity of the recombinant protein?

IBI recombinant proteins are sold in units of micrograms (µg).

The recombinant protein is quantified using the BCA Assay which favors the customer in terms of amount of protein. The BCA assay is not perfect, but at least it is standard for all proteins.

Volume: 25 ug

For more information, please see Specification Sheet