IBI Scientific RPM-170 Recombinant Mouse Protein TNF-alpha 17.3 kDa 5 ug

Recombinant Mouse TNF Alpha Protein (17.3 kDa)

IBI's recombinant mouse TNF alpha protein offers unparalleled quality and performance for your research needs. Produced in a Pichia pastoris (yeast) expression system, this protein benefits from superior folding and post-translational modifications, ensuring enhanced bioactivity compared to those expressed in E. coli systems. Free of endotoxins, HIS-TAGS, and carriers, it is naturally folded and mimics its host form with remarkable fidelity. With a predicted molecular weight of 17.3 kDa, this recombinant protein provides consistency and reliability for diverse applications.

TNF alpha, a critical member of the TNF Superfamily, is known for its pivotal role in regulating immune responses. It is chiefly produced by activated macrophages, alongside various other cell types such as lymphoid cells, mast cells, and fibroblasts. This endogenous pyrogen is involved in key biological processes, including fever induction, apoptosis, inflammation, and the inhibition of tumorigenesis. Shipped lyophilized at room temperature without carrier protein, IBI's TNF alpha mouse protein is ideal for cell culture, use as an ELISA standard, or as a Western Blot control.

Technical Information:

What is an acceptable carrier protein for recombinant protein reconstitution?

A carrier protein is an additional protein added to the lyophilized recombinant protein during reconstitution to increase the stability of the diluted recombinant protein during storage, freezing, and use.

Bovine Serum Albumin (BSA) is the most common carrier protein used for recombinant protein reconstitution. However, if working with bovine recombinant protein, use a non-bovine carrier protein such as Equine Serum Albumin (ESA). 

If using the recombinant protein for in vitro cell culture stimulation, it is often useful to reconstitute the recombinant protein directly in the complete cell culture medium containing serum or other acceptable carrier protein instead of a BSA/ESA solution.

How do I reconstitute the recombinant protein?

Most of the recombinant proteins can be reconstituted in sterile phosphate-buffered saline with 0.1% carrier protein, which is most commonly Bovine Serum Albumin (BSA).

If protein vial is left unopened, it can be stored at room temperature or -20°C for up to 12 months. 

Once protein is open and reconstituted, it must be stored at -20°C and is viable for up to 3 months. 

Avoid repeated freeze/thaw cycles as this could cause denaturation of proteins.

Typically, 100 µL is an acceptable reconstitution volume for 5 µg and 25 µg vials of recombinant protein. IBI's 5 µg and 25 µg vials have a maximum volume of 500 µL.

Typically, 1 mL is an acceptable reconstitution volume for 100 µg vials of recombinant protein. IBI's 100 µg vials have a maximum volume of 2 mL

What concentration of carrier protein is required for reconstitution?

The concentration of carrier protein should be at least 0.1% in the final solution. Additional carrier protein can be used, if desired. Typically, a 0.1-10% BSA solution in 1X PBS is an acceptable reconstitution solution.

What concentration is the recombinant protein upon reconstitution?

The concentration of recombinant protein is dependent on the reconstitution volume. For example, if reconstituting 5 µg of recombinant protein in 100 µL carrier protein solution, the concentration is 5 µg/100 µL or 50 µg/mL

What is the activity of the recombinant protein?

IBI recombinant proteins are sold in units of micrograms (µg).

The recombinant protein is quantified using the BCA Assay which favors the customer in terms of amount of protein. The BCA assay is not perfect, but at least it is standard for all proteins.

Volume: 5 ug

For more information, please see Specification Sheet