IBI Scientific RPH-150 Recombinant Human Protein APRIL 16.4 kDa 5 ug

Superior Quality Recombinant Protein with 16.4 kDa Molecular Weight

The IBI recombinant protein, with a predicted molecular weight of 16.4 kDa, is crafted for research excellence. Produced in the advanced Pichia pastoris yeast expression system, this protein is naturally folded and undergoes critical post-translational modifications, ensuring unmatched bioactivity and structural integrity. Unlike traditional E. coli systems, this method eliminates endotoxins, HIS-TAGS, and any other tags, resulting in a highly pure and functional product. Shipped lyophilized and carrier-free, it offers ease of reconstitution in sterile PBS, tailored for diverse scientific applications.

Engineered for Precision and Reliability

IBI's yeast-based production system enhances the activity and host-like characteristics of its recombinant proteins, ensuring optimal performance in experimental setups. With its high purity and endotoxin-free nature, this protein is ideal for immunological research, protein analysis, and biochemical studies. Backed by rigorous quality standards and proudly made in the USA, IBI recombinant proteins are trusted by researchers for their accuracy and reliability. For technical specifications and additional insights, explore the detailed product information below.

Technical Information:

What is an acceptable carrier protein for recombinant protein reconstitution?

A carrier protein is an additional protein added to the lyophilized recombinant protein during reconstitution to increase the stability of the diluted recombinant protein during storage, freezing, and use.

Bovine Serum Albumin (BSA) is the most common carrier protein used for recombinant protein reconstitution. However, if working with bovine recombinant protein, use a non-bovine carrier protein such as Equine Serum Albumin (ESA). 

If using the recombinant protein for in vitro cell culture stimulation, it is often useful to reconstitute the recombinant protein directly in the complete cell culture medium containing serum or other acceptable carrier protein instead of a BSA/ESA solution.

How do I reconstitute the recombinant protein?

Most of the recombinant proteins can be reconstituted in sterile phosphate-buffered saline with 0.1% carrier protein, which is most commonly Bovine Serum Albumin (BSA). 

If protein vial is left unopened, it can be stored at room temperature or -20°C for up to 12 months. 

Once protein is open and reconstituted, it must be stored at -20°C and is viable for up to 3 months. 

Avoid repeated freeze/thaw cycles as this could cause denaturation of proteins.

Typically, 100 µL is an acceptable reconstitution volume for 5 µg and 25 µg vials of recombinant protein. IBI's 5 µg and 25 µg vials have a maximum volume of 500 µL. 

Typically, 1 mL is an acceptable reconstitution volume for 100 µg vials of recombinant protein. IBI's 100 µg vials have a maximum volume of 2 mL

What concentration of carrier protein is required for reconstitution?

The concentration of carrier protein should be at least 0.1% in the final solution. Additional carrier protein can be used, if desired. Typically, a 0.1-10% BSA solution in 1X PBS is an acceptable reconstitution solution.

What concentration is the recombinant protein upon reconstitution?

The concentration of recombinant protein is dependent on the reconstitution volume. For example, if reconstituting 5 µg of recombinant protein in 100 µL carrier protein solution, the concentration is 5 µg/100 µL or 50 µg/mL

What is the activity of the recombinant protein?

IBI recombinant proteins are sold in units of micrograms (µg).

The recombinant protein is quantified using the BCA Assay which favors the customer in terms of amount of protein. The BCA assay is not perfect, but at least it is standard for all proteins. 

Volume: 5 ug

For more information, please see Specification Sheet