IBI Scientific RPH-135 Recombinant Human Protein IL-8 9.1 kDa 100 ug - 5 pcs

Predicted Molecular Weight: 9.1 kDa

The recombinant human IL-8 protein is meticulously designed to achieve a predicted molecular weight of 9.1 kDa, ensuring consistent and reliable results in research applications such as cell culture, ELISA standards, and Western blot controls. This lyophilized protein is shipped at room temperature without carrier proteins, maintaining its stability and activity during transport. Reconstitution is simple-dissolve in sterile PBS with at least 0.1% carrier protein like BSA or cell assay media to ensure optimal performance in experimental workflows.

No Endotoxins, TAGS, and Carrier-Free

IBI recombinant IL-8 proteins are produced using the Pichia pastoris (yeast) system, offering distinct advantages over traditional E. coli systems. This cutting-edge approach ensures proper protein folding and post-translational modifications for enhanced bioactivity. Endotoxin-free, HIS-TAGS-free, and carrier-free, this recombinant protein delivers exceptional purity and functionality. IL-8, a critical chemokine in the CXC family, plays a vital role in neutrophil migration through its interaction with CXCR1 and CXCR2. Its high activity and structural integrity make it ideal for studies in immunology and chemokine biology.

Technical Information:

What is an acceptable carrier protein for recombinant protein reconstitution?

A carrier protein is an additional protein added to the lyophilized recombinant protein during reconstitution to increase the stability of the diluted recombinant protein during storage, freezing, and use.

Bovine Serum Albumin (BSA) is the most common carrier protein used for recombinant protein reconstitution. However, if working with bovine recombinant protein, use a non-bovine carrier protein such as Equine Serum Albumin (ESA). 

If using the recombinant protein for in vitro cell culture stimulation, it is often useful to reconstitute the recombinant protein directly in the complete cell culture medium containing serum or other acceptable carrier protein instead of a BSA/ESA solution.

How do I reconstitute the recombinant protein?

Most of the recombinant proteins can be reconstituted in sterile phosphate-buffered saline with 0.1% carrier protein, which is most commonly Bovine Serum Albumin (BSA). 

If protein vial is left unopened, it can be stored at room temperature or -20°C for up to 12 months. 

Once protein is open and reconstituted, it must be stored at -20°C and is viable for up to 3 months. 

Avoid repeated freeze/thaw cycles as this could cause denaturation of proteins.

Typically, 100 µL is an acceptable reconstitution volume for 5 µg and 25 µg vials of recombinant protein. IBI's 5 µg and 25 µg vials have a maximum volume of 500 µL. 

Typically, 1 mL is an acceptable reconstitution volume for 100 µg vials of recombinant protein. IBI's 100 µg vials have a maximum volume of 2 mL

What concentration of carrier protein is required for reconstitution?

The concentration of carrier protein should be at least 0.1% in the final solution. Additional carrier protein can be used, if desired. Typically, a 0.1-10% BSA solution in 1X PBS is an acceptable reconstitution solution.

What concentration is the recombinant protein upon reconstitution?

The concentration of recombinant protein is dependent on the reconstitution volume. For example, if reconstituting 5 µg of recombinant protein in 100 µL carrier protein solution, the concentration is 5 µg/100 µL or 50 µg/mL

What is the activity of the recombinant protein?

IBI recombinant proteins are sold in units of micrograms (µg).

The recombinant protein is quantified using the BCA Assay which favors the customer in terms of amount of protein. The BCA assay is not perfect, but at least it is standard for all proteins. 

Volume: 100 ug - 5 pcs

For more information, please see Specification Sheet