IBI Scientific RPH-111 Recombinant Human Protein TNF-alpha 16.8 kDa 25 ug

Predicted Molecular Weight: 17.2 kDa

The recombinant human TNF alpha protein has a predicted molecular weight of 17.2 kDa, ensuring precision and reliability for various research applications, including cell culture, ELISA standards, and Western blot controls. This lyophilized protein is shipped at room temperature without carrier proteins, preserving its activity and stability during transport. To prepare for use, the protein can be reconstituted in sterile PBS with at least 0.1% carrier protein, such as BSA or cell assay media, ensuring maximum performance in your experiments.

No Endotoxins, TAGS, and Carrier-Free

IBI recombinant proteins, including TNF alpha, are produced using the Pichia pastoris (yeast) system, which offers significant advantages over E. coli systems. This process ensures proper protein folding and post-translational modifications, enhancing bioactivity and functional integrity. TNF alpha, a key cytokine in immune regulation, is involved in processes such as fever induction, inflammation, apoptotic cell death, and tumorigenesis inhibition. With its exceptional purity and functionality, this recombinant TNF alpha protein is an essential tool for research in immunology, oncology, and infectious disease studies.

Technical Information:

What is an acceptable carrier protein for recombinant protein reconstitution?

A carrier protein is an additional protein added to the lyophilized recombinant protein during reconstitution to increase the stability of the diluted recombinant protein during storage, freezing, and use.

Bovine Serum Albumin (BSA) is the most common carrier protein used for recombinant protein reconstitution. However, if working with bovine recombinant protein, use a non-bovine carrier protein such as Equine Serum Albumin (ESA).

If using the recombinant protein for in vitro cell culture stimulation, it is often useful to reconstitute the recombinant protein directly in the complete cell culture medium containing serum or other acceptable carrier protein instead of a BSA/ESA solution.

How do I reconstitute the recombinant protein?

Most of the recombinant proteins can be reconstituted in sterile phosphate-buffered saline with 0.1% carrier protein, which is most commonly Bovine Serum Albumin (BSA).

If protein vial is left unopened, it can be stored at room temperature or -20°C for up to 12 months. Once protein is open and reconstituted, it must be stored at -20°C and is viable for up to 3 months.

Avoid repeated freeze/thaw cycles as this could cause denaturation of proteins.

Typically, 100 µL is an acceptable reconstitution volume for 5 µg and 25 µg vials of recombinant protein. IBI's 5 µg and 25 µg vials have a maximum volume of 500 µL.

Typically, 1 mL is an acceptable reconstitution volume for 100 µg vials of recombinant protein. IBI's 100 µg vials have a maximum volume of 2 mL

What concentration of carrier protein is required for reconstitution?

The concentration of carrier protein should be at least 0.1% in the final solution. Additional carrier protein can be used, if desired. Typically, a 0.1-10% BSA solution in 1X PBS is an acceptable reconstitution solution.

What concentration is the recombinant protein upon reconstitution?

The concentration of recombinant protein is dependent on the reconstitution volume. For example, if reconstituting 5 µg of recombinant protein in 100 µL carrier protein solution, the concentration is 5 µg/100 µL or 50 µg/mL

What is the activity of the recombinant protein?

IBI recombinant proteins are sold in units of micrograms (µg).

The recombinant protein is quantified using the BCA Assay which favors the customer in terms of amount of protein. The BCA assay is not perfect, but at least it is standard for all proteins.

Activity cannot be determined for the animal recombinant proteins because an assay does not exist. (Only human proteins have an assay to determine activity.

If end-user wants to know the activity, they can do an activity titer for each lot they use. This is useful because there can be significant differences in activity from lot to lot.

Volume: 25 ug

For more information, please see Specification Sheet