IBI Scientific IB74050 Methylene Blue 25 g

Methylene Blue is a recommended stain for agarose gels when recovery of DNA is required. The benefit of using methylene blue (rather than ethidium bromide) is the ability to use a white light box for visualization and photography in place of a UV light box. Using a white light box eliminates mutations that may affect the integrity of the isolated DNA.

Recommended Use:

Methylene Blue is a recommended stain for agarose gels when recovery of DNA is required. The benefit of using Methylene Blue (rather than ethidium bromide) is the ability to use a white light box for visualization and photography in place of a UV light box. Using a white light box eliminates mutations that may affect the integrity of the isolated DNA.

• Stain a 1% gel for 15 minutes with 0.02% Methylene Blue Solution in distilled water.
• De-stain with distilled water for 15 minutes.

Used for staining RNA that is bonded to nylon and nitrocellulose membranes.

• Soak the dried filter in 5% acetic acid at room temperature for 15 minutes.
• Place the filter in 0.5 M Sodium Acetate (pH 5.2) and 0.04% Methylene Blue for 5-10 minutes at room temperature.
• Rinse the filter in water for 5-10 minutes.

Purity of Methylene Blue:

The purity of methylene blue is stated as Dye Content as measured on a dry basis. The Dye Content for IBI Methylene Blue is 98% - 103%.

For more information, please see Specification Sheet and SAFETY DATA SHEET