IBI Scientific IB53000B MP-1015 Multi Purpose Horizontal Electrophoresis System Lab Start-Up Kit

High-Performance Horizontal Electrophoresis System

IBI's MP-1015 Multi-Purpose Gel Box (10 x 15 cm) is a general-purpose unit designed for both extended high-resolution linear runs and economical quick screens. This Horizontal Electrophoresis system is equipped with recirculation ports for procedures requiring buffer recirculation. The MP-1015 IBI electrophoresis unit is constructed of durable cast acrylic, ensuring it withstands high voltages and extreme temperatures without cracking or warping, unlike most molded units.

The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the MP-1015 is 140 V. In a 1% TBE gel this translates into a run time of approximately one hour. Lower voltages may be used and as a general rule a 70 V run will take twice as long as a 140 V run. Higher voltages may be used to decrease run time. However, if the unit is being operated at higher voltages than 140 V the heat generated during electrophoresis may decrease sample resolution. Such artifacts may be avoided by running the unit in a cold room, recirculating buffer, or adding 1 X electrophoresis buffer "ice cubes" to keep the unit properly cooled. The suggested run parameters for the electrophoresis of RNA in agarose gels containing formaldehyde is 60-80 V.

Characteristics of Horizontal Electrophoresis Units:

IBI horizontal systems are constructed of 3/8" thick cast acrylic, the heaviest in the industry. No warping occurs when the system gets hot, and there are no crazy cracks in the plastic after time. The buffer tank on the IBI systems can be completely re-built, by the end user, in minutes. IBI horizontal systems have a 4-year limited warranty on the buffer tank. IBI horizontal systems allow the user to cast gels OUTSIDE the buffer tank, this is much easier and allows user to cast a gel while a gel is running. IBI systems are 20% less expensive and come with casting system and combs. This info applies to all of IBI horizontal systems against nearly all of other competitors systems.

Start-Up Kit Includes:

• IB53000 Multi-Purpose unit
• 6X loading dye
• 10X TAE buffer
• 10X TBE buffer
• 100gm of molecular biology grade agarose

For more information, please see Manual