IBI Scientific IB49142 Terrific Broth 10 kg

IBI's Terrific Broth is used with Glycerol in cultivating recombinant strains of E. coli. Terrific broth is a highly enriched medium for improving yield in plasmid bearing E. coli. Recombinant strains have an extended growth phase in the medium. The addition of tryptone and yeast extract in the medium will allow higher plasmid yield per volume.

Formula per Liter:

• Casein Digest Peptone 12.0 gm
• Yeast Extract 24.0 gm
• Potassium Phosphate, Monobasic 2.3 gm
• Potassium Phosphate, Dibasic 12.5 gm

Preparation:

• Mix 50.8 gm of the medium in 1 L of purified water until evenly dispersed.
• Add 4 ml of Glycerol to the mixture.
• Heat with repeated stirring, and boil for one minute to dissolve completely.
• Distribute and autoclave at 121.0°C for 15 minutes.

Storage:

Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning:

Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.

Size: 10 kg

For more information, please see Specification Sheet and SAFETY DATA SHEET