IBI Scientific IB47640 RNA Pure Kit (4 Reactions)

The RNA Pure Kit uses a simple and efficient spin column procedure to purify Total RNA stored in RNase-free water, elution buffer or TE Buffer following extraction using acid-guanidinium-phenol-chlorofom based methods such as TRIzol® Reagent and IBI Isolate. Contaminants such as RNases, DNA and residual phenol are effectively removed using a simple 4 step procedure. The high-quality, total RNA is eluted in RNase-free Water or TE (RNase-free) and is ready for use in a variety of sensitive downstream applications.

Features:

• Sample Size: 50 μg of total RNA
• Operation time: 10 minutes
• Recovery: Up to 80% of high quality RNA 
• Elution Volume: 20 - 50 μl
• Compatibility: Purify RNA stored in RNase-free water, elution buffer or TE Buffer following extraction using IBI Isolate TRI-Reagent®, TRIzol®, RNAzol® and QIAzol® etc.
• Applications: RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection Assay
• Additional Requirements: 1.5 ml microcentrifuge tubes, absolute ethanol

Technical Information

The extracted RNA can be used directly in a variety of downstream applications such as RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection Assay.

Quality Control

The RNA Pure Kit is tested on a lot-to-lot basis. Following RNA purification using the RNA Pure Kit, 10 μl from a 50 μl eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

This kit has 4 reactions.

For more information, please see Protocol