IBI Scientific IB47300 Mini Total RNA Kit for Tissue (4 Preps, Sample Kit)

The Total RNA Mini Kit for Tissue was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase (optional in-column DNase treatments can be followed). RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column. Once any contaminants have been removed , using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free water. The procedure does not require phenol extraction or alcohol precipitation and can be completed within 15 minutes. The purified RNA is ready-for-use in RT-PCR, northern blotting, primer extension, and cDNA library construction.

The quality of the Total RNA Mini Kit for Tissue is tested on a lot-to-lot basis according to IBI's ISO-certified quality management system. Total RNA is isolated from a 25 mg animal tissue sample, quantified with a spectrophotometer, and analyzed by electrophoresis.

Features:

• Sample Size: up to 25mg tissue/up to 25mg paraffin-embedded tissue
• Operation Time: 15min or less
• Expected Yield: 5 - 30µg RNA
• Elution Volume: 25-100µl

This kit has 4 preps.

For more information, please see Protocol and Safety Data Sheet