IBI Scientific IB47291 gBAC Mini Genomic DNA Kit (100 Preps)

The gBAC Mini Genomic DNA Kit has been optimized for purifying total DNA (including genomic and viral DNA) from gram negative and gram positive bacterial cells. The provided gram positive buffer, when combined with lysozyme, will efficiently lyse bacterial cell walls consisting of peptidoglycan.

Chaotropic salt is used to further lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Contaminants are removed using a wash buffer (containing ethanol) and the purified genomic DNA is eluted using a low salt elution buffer or TE. The entire procedure can be completed in one hour without phenol-chloroform extraction or alcohol precipitation. The purified DNA is suitable for PCR and other enzymatic reactions.

The quality of the gBAC Mini DNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from E. coli (1x10⁹) culture (OD600=1.3, 1 ml) harvested by centrifugation at 16,000 xg for one minute. 10 µl from a 50 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

Additional Details:

• Sample Size: up to 1x10⁹ gram (+) or gram (-) bacterial cells
• Expectant Yield: up to 30 µg DNA
• Operation Time: 30 min or less
• Elution Volume: 50-200 µl

This kit has 100 preps.

For more information, please see Protocol