IBI Scientific IB47272 10 x 96-Well Genomic DNA Kit (Plant)

The 10 x 96-Well Genomic DNA Kit (Plant) provides an efficient method for purifying total DNA (including genomic, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are disrupted by both grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer coupled with chaotropic salt, the genomic DNA in the lysate binds to each column of the binding plate. The contaminants are removed with a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The procedure does not require DNA phenol extraction or alcohol precipitation and can be completed in 1 hour. The purified genomic DNA is ready for use  in PCR, Real-time PCR, Southern Blotting and RFLP.

Quality Control

The quality of the 96-Well Genomic DNA Kit (Plant) is tested on a lot-to-lot basis by isolating genomic DNA from a 50
mg young leaf sample. More than 10 μg of genomic DNA is quantified with a spectrophotometer and checked by
electrophoresis.

For more information, please see Protocol