IBI Scientific IB47230 Mini Genomic DNA Kit for Plant/Fungi (4 Preps, Sample Kit)

The Genomic DNA Mini Kit for Plants and Fungi provide a fast and economical method to isolate total DNA (genomic DNA, mitrochondrial, and chloroplast) from plant tissue and cells. Samples are disrupted both by grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and filtered to remove cell debris and salt precipitates. In the presence of the binding buffer coupled with chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix of the spin column. The contaminants are washed with an ethanol based wash buffer. The genomic DNA is then eluted by a low salt elution buffer or water. The protocol does not require DNA phenol extraction or alcohol precipitation. This entire procedure can be completed in 30 minutes or less and the purified genomic DNA is ready for PCR, Real-Time PCR, Southern Blotting, or RFLP.

This kit has 4 preps.

The quality of the Genomic DNA Mini Kit for Plant is tested on a lot-to-lot basis by isolating genomic DNA from 50mg young leaf samples. More than 10µg of genomic DNA is quantified with a spectrophotometer and checked by electrophoresis.

Features:

• Sample Size: up to 100 mg fresh plant tissue/25 mg dry plant tissue
• Expectant Yield: 3-5 μg (100 mg Arabidopsis thaliana leaf), 20-25 μg (100 mg Nicotiana tabacum leaf)
• Operation Time: 30 min or less
• Elution Volume: 30-200µl
• Binding Capacity: 50µg
• Free sample available upon request

For more information, please see Protocol