IBI Scientific IB47153 2 x 96 Well Plasmid Kit

The 2 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 - 5 ml of cultured bacterial cells per/well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit requires a centrifuge or a vacuum manifold capable of running 96 well plates. IBI offers IB47500 96 well vacuum manifold.

Typical yields are 20 - 35 µg for high-copy number plasmid and 3 - 10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions. Click on the tabs above to review the protocol or detailed specifications.

For more information, please see Protocol