IBI Scientific IB06080 Spin Column for the Purification of Radiolabeled (DNA NU-CLEAN R50, Sephadex G50 w/STE) - 10 pcs

The NU-CLEAN R50, Sephadex G-50 spin columns are intended for use in desalting and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from end-label, fill-in, nick-translation, or random primed DNA labeling reactions. This column should be used to purify DNA greater than 72 base pairs in length or RNA greater than 72 base length; smaller DNAs or RNAs will be retained in the column material. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume. For optimum sample recovery and purification when preparing biotinylated probes, use NU-CLEAN spin columns with the "B" designation.

Each DNASE- and RNASE-free column is prepackaged with Sephadex G-50 in sterile STE buffer (10mM Tris HCL, 1mM EDTA, pH 8.0). Two autoclavable collection tubes and one non-autoclavable cap are supplied for each column.

The optimal sample loading volume is 50μl (100μl Max.) with a maximum of 100μg of nucleic acid per column. Sample should not be viscous prior to loading.

For more information, please see Protocol