IBI Scientific IB06045 Microcentrifuge Spin Column (100 uL) for the Purification of Radiolabeled DNA/RNA (MAXI Select-D w/G50 & STE) - 50 pcs

The MAXI SELECT-D STE, G-50 spin columns are intended for use in desalting and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from end-label, fill-in, nick-translation, or random primed DNA labeling reactions. This column should be used to purify DNA greater than 72 base pairs in length or RNA greater than 72 base length; smaller DNAs or RNAs will be retained in the column material. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.

Each DNASE- and RNASE-free column is prepackaged with Sephadex G-50 in sterile STE buffer (10mM Tris HCL, 1mM EDTA, pH 8.0). Two autoclavable collection tubes and one non-autoclavable cap are supplied for each column.
The optimal sample loading volume is 50μl (100μl Max.) with a maximum of 100μg of nucleic acid per column. Sample should not be viscous prior to loading.

For more information, please see Protocol