IBI Scientific IB06020 Microcentrifuge Spin Column (50 uL) for the Purification of Radiolabeled DNA, RNA (MIDI Select-D w/G50 & STE) - 50 pcs

Each DNASE- and RNASE-free column is prepackaged with Sephadex G-50 in sterile STE buffer (10mM Tris HCL, 1mM EDTA, pH 8.0). Two nuclease-free collection tubes (autoclavable) are supplied for each column. Proper precautions should be taken to avoid contamination of the column, column contents, collection tubes and samples with exogenous RNASE. The supplied columns and collection tubes are sterile and nuclease-free.

The optimal sample loading volume is 50μl with a maximum of 50μg of nucleic acid per column. Sample should not be viscous prior to loading.

For best results use a microcentrifuge with a fixed angle or horizontal rotor capable of 12,000-16,000 x g. Please note that the microcentrifuge must be suitable for use with 2ml microcentrifuge tubes. In particular, the 2ml tubes positioned in the rotor must not contact any part of the microcentrifuge interior. Use of a closed-bottom rotor compatible of accepting 2ml tubes is recommended.

Recommended Use

The MIDI SELECT-D STE, G-50 microcentrifuge spin columns are intended for use in desalting, recovering DNA fragment (>72bp, smaller DNAs will be retained), and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from small volume 5' end-labeling reactions and fill-in labeling reactions utilizing a DNA polymerase. In addition, the MIDI SELECT-D STE, G-50 spin columns are RNASE-free and can be used for the rapid purification of RNA (>72bases) away from unincorporated ribonucleotides (rNTPs) and in other related applications. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume

G50 Sephadex inside spin column containing frit filter. Column capped on both ends.

For more information, please see Protocol