IBI Scientific IB06015 Microcentrifuge Spin Column (50 uL) for the Purification of Radiolabeled DNA & RNA (MIDI Select-D w/G50 & TE) - 50 pcs

The Midi Select-D, G-50 microcentrifuge spin columns are intended for use in desalting, recovering DNA fragments (>72bp, smaller DNAs will be retained), and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from small volume 5' end-labeling reactions and fill-in labeling reactions utilizing a DNA polymerase. In addition, the Midi Select-D G-50 spin columns are RNase-free and can be used for the rapid purification of RNA (>72 bases) away from unincorporated ribonucleotides (rNTPs) and in other related applications. After brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.

Each DNase- and RNase-free column is prepackaged with Sephadex G-50 in sterile TE buffer. Two nuclease-free collection tubes (autoclavable) are supplied for each column.

The optimal sample loading volume is 50µl with a maximum of 50µg of nucleic acid per column.

• Retention of unincorporated NTPs: >95%
• Recovery of DNA (>72bp): >90%
• Recovery of RNA (>72 bases): >80%
• Operation time: up to 10 minutes
• DNase- and RNase-free

G50 Sephadex inside spin column containing frit filter. Column capped on both ends.

For more information, please see Protocol