The Midi Select-D TE, G-25 microcentrifuge spin columns are intended for use in desalting, recovering DNA fragments (>12mer), and removing unincorporated radiolabeled deoxynucleoside triphosphates (dNTPs) from small volume 5' end-labeling reactions and fill-in labeling reactions utilizing a DNA polymerase. In addition, the Midi Select-D TE, G-25 spin columns are RNase-free and can be used for rapid purification of oligoribonucleotides and RNA from unincorporated ribonucleotides (rNTPs) and in other related applications. After a brief centrifugation, the purified nucleic acid is recovered from the column without significant change in volume.
G25 Sephadex inside spin column containing frit filter. Column capped on both ends.
Each DNase- and RNase-free column is prepackaged with Sephadex G-25 in sterile TE buffer. Two nuclease-free collection tubes (autoclavable) are supplied for each column.
- Retention of unincorporated NTPs: up to 90%
- Operation time: up to 10 minutes
- DNase- and RNase-free
For more information, please see Protocol