IBI Scientific IB05406 Proteinase K Solution 5 mL

IBI Scientific Recombinant Proteinase K Solution contains Recombinant Proteinase K powder, originally isolated from the mold Engyodontium album, is a serine protease with broad substrate specificity and relatively high proteolytic activity. It preferentially cleaves ester and peptide bonds adjacent to the C-termini of hydrophobic, aliphatic, or aromatic amino acids. 

IBI Scientific recombinant Proteinase K Powder is highly pure, determined by SDS-PAGE to be 95%.

High purity, sterility, no bio-burden and no presence of DNase, RNase, DNA, and RNA contaminants.

By using absorbance A275 as the vertical axis and different concentrations of a tyrosine as the horizontal axis the enzymatic activity was calculated to >30U/mg.

IBI Scientific recombinant Proteinase K Powder is expressed in pichia pastoris, purified and lyophilized. 

How This Solution Was Manufactured:

• 50mM Tris
• 3mM CaCl2
• 50% Glycerol
• Adjust pH to 8.0 with either HCl or NaOH
• Dissolve solids in 50% volume of Certified Nuclease Free Water (IB42100)
• QS to 95% with glycerol. pH to 8.0 using HCL or NaOH.
• QS to 100% volume with glycerol.
• Recheck pH using different meter.
• Mix well using a sterile stir bar and filter through a 0.2 micron sterile filter.
• Store buffer at 4C and let chill before preparing Proteinase K solution.

Using cold buffer, remove Proteinase K from freezer and dilute powder to 10mg,mL with buffer. Mix well using a stir bar. Filter solution through a 1.2 micron PES membrane syringe or disc filter and aseptically fill into final container. Aliquot and store at -20C. 

Storage:

• -20 °C for up to 2 years
• 4C for up to 18 months
• Room temperature for up to 12 months
• Is best to make aliquots for multiple uses
• Avoid repeated freeze-thaw cycles

For more information, please see Specification Sheet and SAFETY DATA SHEET