IBI Scientific IB05402 Proteinase K Powder 200 mg

Highly Pure IBI Scientific Recombinant Proteinase K Powder:

IBI Scientific Recombinant Proteinase K Powder offers unmatched quality and performance for a wide range of molecular biology applications. Derived from the mold Engyodontium album and expressed in Pichia pastoris, this high-purity serine protease boasts 95% purity as confirmed by SDS-PAGE. Designed for broad substrate specificity, Proteinase K cleaves ester and peptide bonds near the C-termini of hydrophobic, aliphatic, or aromatic amino acids, ensuring efficient protein digestion. With sterility and an absence of DNase, RNase, DNA, and RNA contaminants, this product provides exceptional reliability for downstream applications. Each batch of Proteinase K Powder is lyophilized to maintain stability and ease of use, meeting the stringent demands of research and diagnostic settings.

Superior Performance and Enzymatic Activity of Proteinase K:

IBI Scientific Recombinant Proteinase K Powder delivers exceptional enzymatic activity, measured at over 30 U/mg using absorbance A275 against tyrosine standards. This ensures precise and efficient protein degradation, crucial for DNA and RNA purification workflows. Its sterility and absence of bio-burden make it an ideal choice for sensitive applications. Whether you're working in genomics, proteomics, or microbiology, IBI Scientific Proteinase K Powder is optimized for consistent performance. Choose IBI Scientific to experience superior quality and reliability in your proteinase needs, supporting your research with cutting-edge precision.

To place this product into solution:

• 50mM Tris
• 3mM CaCl2
• 50% Glycerol
• Adjust pH to 8.0 with either HCl or NaOH
• Dissolve solids in 50% volume of Certified Nuclease Free Water (IB42100)
• QS to 95% with glycerol. pH to 8.0 using HCL or NaOH.
• QS to 100% volume with glycerol.
• Recheck pH using different meter.
• Mix well using a sterile stir bar and filter through a 0.2 micron sterile filter.
• Store buffer at 4C and let chill before preparing Proteinase K solution.

Using cold buffer, remove Proteinase K from freezer and dilute powder to 10mg,mL with buffer. Mix well using a stir bar. Filter solution through a 1.2 micron PES membrane syringe or disc filter and aseptically fill into final container. Aliquot and store at -20C. 

• Use containers and materials that have low protein binding characteristics. 

Note: If you are able to use this product entirely in 15 days or less, IBI Recombinant Proteinase K Powder can be placed directly into Pure water and 50% glycerin at required concentration. 

Storage:

• - 20 °C for up to 2 years
• 4C for up to 18 months
• Room temperature for up to 12 months
• Is best to make aliquots for multiple uses
• Avoid repeated freeze-thaw cycles

Size: 200 mg

For more information, please see Specification Sheet and SAFETY DATA SHEET