IBI Scientific IB02150 IPTG / X-GAL Spray Solution 20 mL

Ready-to-Use IPTG/X-GAL Solution for Cloning Selection

IBI's IPTG/X-GAL Solution is the perfect tool for selecting cloning constructs in LacZ expression systems involving the lacI repressor. This ready-to-use product comes in an easy-to-use spray bottle, making application simple and efficient. With a concentration of 8 mg/ml IPTG and X-GAL, each pump delivers approximately 0.20 ml of solution, ensuring precise and consistent application. Spread the solution evenly on the plate, allow 5-10 minutes for absorption, and experience enhanced results thanks to proprietary formulation, which intensifies blue color contrast and reduces false positives for effortless colony picking.

Enhanced Stability and Performance

IBI's IPTG/X-GAL Solution is uniquely formulated to remain stable at room temperature for extended periods, making it more versatile than other solutions on the market. For long-term storage, refrigeration at 4°C is recommended. Additionally, this solution is made without DMSO, ensuring safe and effective use. Its enhanced blue color improves colony visibility and reduces the chances of errors, making it an indispensable tool for molecular cloning workflows. Choose IBI's solution for reliable, high-performance colony selection in every experiment.

Solution Components: Isopropyl -D-thiogalatopyranoside 5' bromo-4-chloro-3-indolyl-D-galactopyranoside

Appearance: Clear, colorless to yellow solution

Does not contain toxic DMF and DMSO.

Recommended Use

IBI's IPTG / X-GAL Solution is a ready-to-use product packaged in an easy-to-use spray bottle. For use in selection of cloning construct in LacZ expression in the presence of lacl repressor. The concentration of this solution is 8 mg/ml of IPTG and X-GAL. Each pump of the easy to use spray bottle produces approximately 0.20 ml of solution. This volume of solution per/actuation should be used to determine your required volume per plate. Spread solution and pause to ensure absorption, waiting approximately 5 to 10 minutes.

Storage

Store at +4°C. Keep tightly sealed.

For more information, please see Specification Sheet and SAFETY DATA SHEET