IBI Scientific IB02100 IPTG (Isopropyl Beta-D-thiogalatopyranoside) 1 g

IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications to allow detection of recombinant molecules. Size: 1 g.

Recommended Use

IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications, thus allowing detection of recombinant molecules.
Prepare a stock solution of IPTG at 0.1 M (23.8 mg/mL) in sterile distilled water and filter sterilize through a 0.22 micron disposable filter. To either of the below solutions add 3 ml/L IPTG stock solution. When pouring plates add 15 gm bacto-agar per liter.

Solution 1: 10 x M9 Salts Solution: 60 g Na2HPO4

30 g KH2PO4

5 g NaCl

10 g NH4Cl

Adjust the pH to 7.4, add water to a final volume of 1L and autoclave. For M9 media add: 100 ml 10 x M9 Salt Solution

1 ml 0.01 M CaCl2

2 ml 1.0 M MgSO4

Adjust to a final volume of 1 liter with distilled water. Supplement with (optional): 0.2% Casamino Acids

0.4% Glucose

40 mg/ml Thiamine-HCL

Solution 2: 2 x YT Media: 16 g/L Tryptone

10 g/L Yeast Extract

5 g/L NaCl

Storage

Store desiccated at -20°C. Warm to room temperature before opening. It is useable at room temperature for 36 hours. Keep tightly sealed.

For more information, please see Specification Sheet and SAFETY DATA SHEET