IBI Scientific IB01302 100bp DNA Ladder, 500 uL, No Loading Dye

100 bp DNA Ladder - Reliable Molecular Weight Standard for Electrophoresis

The IBI 100 bp DNA Ladder is an essential tool for researchers conducting molecular biology experiments that require precise DNA fragment sizing. Designed to serve as a molecular weight standard, this ladder provides accurate and reliable sizing of double-stranded DNA fragments ranging from 100 base pairs (bp) to 3,000 bp. With its 12 distinct fragments and enhanced intensity at the 500 bp marker, the 100 bp DNA Ladder ensures high resolution and ease of use in agarose gel electrophoresis.

Exceptional Accuracy and Performance in DNA Analysis

The IBI 100 bp DNA Ladder offers a broad size range, covering DNA fragments from 100 bp to 3,000 bp. This makes it highly versatile for various molecular biology applications, including PCR product verification, plasmid analysis, and genome mapping. The enhanced 500 bp fragment provides a strong reference point, allowing for quick and easy identification of DNA fragment sizes.

This DNA ladder is manufactured through a precise combination of proprietary plasmids digested with restriction enzymes and PCR-generated fragments. The result is a set of well-defined DNA fragments that migrate consistently during electrophoresis, ensuring accuracy in molecular weight determination.

Ideal for Agarose Gel Electrophoresis and Molecular Biology Applications

The IBI 100 bp DNA Ladder is optimized for use in agarose gel electrophoresis, making it a critical tool for molecular biology research. It enables researchers to accurately determine the size of unknown DNA fragments in PCR analysis, restriction digest mapping, and quality control testing of DNA samples. By providing sharp and clearly defined bands, this DNA ladder enhances resolution and ensures high reproducibility in gel electrophoresis experiments.

This ladder is also widely used in quality control applications, allowing scientists to assess the integrity and purity of their DNA samples. It serves as a reliable benchmark in DNA cloning, sequencing preparation, and various diagnostic applications requiring molecular weight determination. The high-quality composition and strict manufacturing standards of this product guarantee dependable results in all experimental workflows.

How to Use the 100 bp DNA Ladder for Optimal Results

Using the IBI 100 bp DNA Ladder is simple and efficient. Researchers can load 5 μL of the ready-to-use ladder directly onto the agarose gel without requiring dilution. The electrophoresis conditions should be optimized based on the gel concentration and buffer system used. After running the gel, the DNA fragments can be visualized using a UV transilluminator or gel imaging system with an appropriate nucleic acid stain such as ethidium bromide or SYBR Safe. Proper handling and storage ensure the ladder's continued accuracy and reliability.

Stable and Long-Lasting Performance with Proper Storage

To maintain the integrity of the 100 bp DNA Ladder, it should be stored properly based on the expected duration of use. Short-term storage at room temperature is sufficient for up to six months. For extended stability, refrigeration at 4°C is recommended for up to one year, while long-term storage at -20°C preserves the ladder for up to two years. Minimizing freeze-thaw cycles will help maintain product quality, ensuring consistent and reproducible performance over time.

Safety and Handling Guidelines for Laboratory Use

The IBI 100 bp DNA Ladder is safe for routine laboratory use and does not pose significant health hazards when handled correctly. Standard laboratory safety protocols should be followed, including the use of personal protective equipment such as gloves and safety goggles. Researchers should also adhere to proper waste disposal guidelines when disposing of used gel materials containing nucleic acids.

For more information, please see Specification Sheet