AmScope T800-FLLED T800 High-performance Multi-band LED-Fluorescence Trinocular Compound Microscope

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AmScope T800-FLLED T800 High-performance Multi-band LED-Fluorescence Trinocular Compound Microscope
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Overview


AmScope T800 High-performance Multi-band LED-Fluorescence Trinocular Compound Microscope

  • 3-band LED-excited fluorescence with multi-filter turret
  • A robust, high-performance microscope with a wide range of contrast-enhancing methods available
  • Infinity-corrected, plan, semi-apochromatic optics with edge-to-edge sharpness
  • Koehler illumination with multi-NA swing-condenser for high and low magnification

Overview

The T800-FLLED upright fluorescence microscope uses a cutting-edge 3-LED illuminator for variable-band excitation. Oufitted with the widely-used green (G1), blue (B1), and ultraviolet (UV2) excitation-bands, it can be used for a wide variety of common labeling or autofluorescence. Unlike single-LED fluorescence illuminators, the three LEDs are cycled using a multi-filter turret, eliminating the need to exchange light-sources. Each LED provides a precise excitation-source to eliminate non-relevant light, as well as providing maximum intensity at the targeted wavelength. Another major benefit of LED illumination is the elimination of long warm-up times and other care required by fluorescent light-sources such as mercury-vapor.

The infinity-corrected optical system includes plan-fluor objectives, which provide edge-to-edge sharpness with higher resolution, improved color-correction, and improved short and long wavelength transmission. The reversed quintuple-nosepiece is equipped with 4X, 10X, 20X, 40X, and 100X plan semi-apochromatic lenses. The 10X plan, high-eyepoint eyepieces provide a compounded magnification range of 40X to 1000X. The Koehler illumination-system is designed for precision, with a variable-NA swing-condenser allowing the use of high and low magnifications with full, even coverage. The robust frame offers excellent stability and ergonomics, with coaxial coarse and fine focusing, as well as coaxial stage-translation knobs providing fluid control.

Fluorescence

Filters
Filter SetChartExcitation FiterDichromatic MirrorEmission FiterCommon Stains
UV2AmScope-T800-FLLED340-390nm400nm420nmAlexa Fluor (350, 405), BFP, DAPI, Dopamine, Hoechst (33258, 33342, 34580)
B1AmScope-T800-FLLED450-490nm495nm500-550nmAcridine Orange (DNA/RNA), Alexa Fluor (488, 500, 514, 610, 647), Chlorophyll, Cy2, DiO, GFP, Rhodamine (110, 123)
G1AmScope-T800-FLLED540-580nm585nm590-665nmTexas Red®, Texas Red® X, Cy3.5, Mito Tracker® Red
LED Excitation
BandPeak Wavelength
UV2385nm
B1470nm
G1560nm

Specifications

Optical SystemInfinity-corrected
HeadTrinocular, 30 ° incline, 360 ° rotatable
Interpupillary AdjustmentCompensation-free gemel, 48-75mm
EyepiecesPlan 10X, high-eyepoint, FN 22mm, 30mm mount
Photo-port1X focus-adjustable C-mount
NosepieceQuintuple reversed
Objective Lenses4X, 10X, 20X, 40X (spring), 100X (spring, oil)
Focusing SystemCoaxial coarse and fine focus, upper limit-stop, tension-control
Focus Travel30mm, 0.002mm fine-adjustment
StageDouble-layer mechanical 185mm x 165mm
X-Y Translation Range80mm x 55mm
Sub-stage Condenser-holderRack-and-pinion, centerable
CondenserAchromatic swing condenser, NA1.2/0.22
Transmitted Illumination3W LED, 6V2A, continous adjustment
Fluorescence Excitation Illumination3 x 3W LED: UV2(385nm), B1(470nm), G1(560nm)

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4XPlan Fluor0.1315.13mm0.17mm
10XPlan Fluor0.308.53mm0.17mm
20XPlan Fluor0.502.33mm0.17mm
40XPlan Fluor0.750.55mm0.17mm
100XPlan Fluor1.280.21mm0.17mm

Components

Objectives
Plan Achromatic Objectives○
Plan Fluor Objectives-
Plan Apochromatic Objectives○
Eyepieces
10X FN22-
15X○
20X○
25X○
Condensers
Brightfield Swing Condenser (NA1.2/0.22)-
Dry Darkfield Condenser (NA0.90)○
Oil Darkfield Condenser (NA1.25)○
Phase-contrast Turret○
Heads
Binocular Head○
Trinocular Head-
Tilting Erect-image Trinocular Head○
Photo-ports
(Trinocular Heads Only)
1X C-mount Adjustable Photo-port-
0.5X C-mount Adjustable Photo-port○
0.3X C-mount Adjustable Photo-port○
23mm Adjustable Photo-port○
- included○ optional- not available

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