AmScope IN480TC-FL Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X Magnification w/ Kohler Illumination

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AmScope IN480TC-FL Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X Magnification w/ Kohler Illumination
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  • Brand:AmScope
  • Part #:IN480TC-FL
  • Model #:IN480 Series
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Overview


AmScope IN480 Series Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X Magnification with Kohler Illumination

  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage™s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm

Specifications

AmScope-IN480TC-FL

Technical drawings with measurements (in mm)


Optical SystemInfinity-corrected
Reference Focal Length220mm
HeadTrinocular, 45° incline
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter30mm
Ocular Dioptersleft ocular
Eyepieces10X, 25X
Photo-portCX-type height-adjustable, 23mm tube
Objective LensesInfinity-corrected
Objective Turretquintuple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
Stagefixed-height, mechanical X-Y translation
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range120mm x 80mm
Specimen HoldersTerasaki plate, Petri dish
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
CondenserNA 0.3, centerable
Condenser Working Distance72mm maximum
Phase Contrast Annuli4X/10X and 20X/40X
Power90-240VAC 50/60Hz wide-band

Objective Lenses

20X
MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
plan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • ⚠️ Attention CA Residents: Prop 65 Warning

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