AmScope IN480T-FL-3MBI3 Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-600X Magnification w/ Kohler Illumination and 3.1MP Back-illuminated CMOS USB 3.0 Camera

AmScope IN480 Series Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-600X Magnification with Kohler Illumination and 3.1MP Back-illuminated CMOS USB 3.0 Camera

• 40X to 600X magnification range using a quintuple lens turret for five unique settings.
• An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
• The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
• The infinity-corrected optical system ensures high-quality, high-resolution images.
• Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
• The trinocular photo port provides a dedicated interface for mounting a camera.

The IN480T-FL is our top-of-the-line inverted epi-fluorescence microscope, accommodating diverse observation methods including brightfield, phase-contrast, and epi-fluorescence. The inverted form and modular stage design allow for a wide range of specimen mounts and containers. A wide range of customizations in the Koehler transmitted illumination system provide flexibility and precise control to allow you to achieve optimal results. The reliable and intuitive design ensure you will spend more time focused on your research.

Optics

The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly precise images. Five brightfield objective lenses provide a wide magnification range from 40X to 600X, with plan-achromatic corrections for high resolution, accurate colors, and edge-to-edge sharpness. Additionally, three phase-ring equipped objective lenses can be used for phase-contrast observation.

Viewing Head

The viewing head has a 45&deg incline and Siedentopf interpupillary adjustment for flexibility and comfort. The CX series photo-port uses interchangeable camera mounts to support a variety of imaging solutions. The included 23mm tube mount accommodates our huge selection of microscopy cameras with 23mm reduction lenses. Several optional mounting adapters are available for C-mount cameras, including mounts with integrated reduction to optimize field-of-view based on sensor formats. The viewing platform is equipped with a sliding shutter to isolate the light path when using a camera, avoiding stray light from the oculars.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. This allows for highly-efficient, optimized illumination for maximum resolution and contrast. The swing-out condenser mount allows the entire condenser assembly to be laterally rotated out of the way for easier specimen placement, and for larger containers. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting for brightfield or for use with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Filters
Filter Set Excitation Center Wavelength / Bandwidth Dichromatic Mirror Cut-on Wavelength Emission Center Wavelength / Bandwidth B1 480nm/30nm 505nm 535nm/40nm Common Stains: Alexa Fluor 488, Cy2, FITC G1 560nm/40nm 600nm 635nm/60nm Common Stains: Texas Red, Cy3.5, Mito Tracker Red
Mercury HBO Excitation
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths. Band Peak Wavelength UV 334nm, 365nm V 405nm B 436nm G 546nm Y 578nm

Specifications

Technical drawings with measurements (in mm)

Optical System	Infinity-corrected
Head	Trinocular, 45° incline
Interpupillary Adjustment	Siedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter	30mm
Eyepieces	10X FN22mm plan wide-field
Photo-port	CX-type height-adjustable, 23mm tube
Objective Turret	quintuple
Focusing System	Focus Adjustment Coaxial Coarse/Fine, adjustable tension Fine Division 0.002mm Coarse Stroke 38mm Fine Stroke 0.2mm
Stage	fixed-height, mechanical X-Y translation
Stage Dimensions	160mm x 250mm stage extension: 70mm x 180mm
Stage X-Y Travel Range	120mm x 80mm
Specimen Holders	Terasaki plate, Petri dish
Transmitted Illumination	6V 30W halogen variable intensity centerable lamp
Condenser	NA 0.3, centerable
Condenser Working Distance	72mm maximum
Phase Contrast Annuli	4X/10X and 20X/40X
Power	90-240VAC 50/60Hz wide-band for international use

Objective Lenses

3.08mm

Magnification	Corrections	NA	Working Distance	Cover-glass Thickness
4X	plan achromatic	0.13	10.4mm	1.5mm
10X	plan achromatic	0.25	7.30mm	1.5mm
20X	plan achromatic	0.40	6.79mm	1.5mm
40X	plan achromatic	0.60	3.08mm	1.5mm
60X	plan achromatic	0.70	1.71mm	1.5mm
10X	phase-ring	0.25	7.30mm	1.5mm
20X	phase-ring	0.40	6.80mm	1.5mm
40X	phase-ring	0.60	1.5mm

Magnification Chart

Eyepiece	Objective
	4X	10X	20X	40X	60X
10X	40X	100X	200X	400X	600X

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