AmScope FM820TA-3MBI3 FM820 Series Epi-fluorescence Trinocular Compound Microscope 40X-1500X Magnification w/ 3MP USB 3.0 C-mount Camera

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AmScope FM820TA-3MBI3 FM820 Series Epi-fluorescence Trinocular Compound Microscope 40X-1500X Magnification w/ 3MP USB 3.0 C-mount Camera
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  • Brand:AmScope
  • Part #:FM820TA-3MBI3
  • Model #:FM820 Series
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Overview


Key Features:

  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 15X eyepieces for ten unique settings.
  • A scalable epi-fluorescence microscope for laboratories and universities with wide-spectrum 100W mercury HBO arc-lamp and four turret-mounted filter blocks
  • Includes ultraviolet, violet, blue, and green excitation filter blocks for popular fluorochromes
  • A robust, high-performance microscope with superior optics and mechanical design
  • Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification
  • The infinity-corrected optical system reduces distortion and improves color accuracy.
  • The Koehler illumination system provides centered, evenly-distributed lighting with a field aperture for maximum contrast and definition.
  • The trinocular head provides a dedicated photo port for mounting a camera.
  • Monitor and capture photos and videos with the included highly-sensitive 3.1MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.

The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a wide magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.

Viewing Head

The microscope's head is designed for precision and comfort. The super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. Compensation-free Siedentopf interpupillary adjustment allows any user to properly align the oculars without readjusting focus. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The photo port is equipped with a 23mm tube for attaching cameras with compensating lenses, and is height-adjustable to match focus with the eyepieces. The port is compatible with our YX series C-mount adapters which can attach directly to C-mount cameras without a compensating lens - ideal for 1" format sensors. The head is set to a 30° incline which is ideal for viewing from a standing position, and it can be rotated 360°.

Optics

The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 1000X. Additional 15X eyepieces expand the maximum magnification to 1500X with 10 unique levels. The turret's reversed orientation provides more working room above the stage. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The 2-layer mechanical stage provides smooth and precise movement for examination of specimen slides, with a large translation range for viewing larger samples. The stage's low-position controls are conveniently placed near the coaxial focus knobs for a streamlined workflow. Examining specimen slides is intuitive and precise for users at any skill level. Symmetrical focus controls are coaxially mounted for fast, intuitive focusing. A height limit can be set to prevent contact damage, as well as presetting optimal focus distance.

Koehler Transmitted Illumination

Built on Koehler illumination principals, the sub-stage lighting provides evenly-distributed light for optimal contrast. The rear-mounted 30W halogen light-source provides 100 CRI illumination with ample brightness for contrast-enhancement methods like darkfield. The lower color temperature of the halogen light-source causes less eyestrain compared to high-Kelvin LED. The light condenser uses a swing mechanism for optimized lighting at both high and low magnifications, with a NA0.25 setting for full coverage at extremely low magnifications. An iris diaphragm allows you to adjust the aperture to optimize contrast and depth-of-field. The condenser holder uses rack-and-pinion height adjustment for precision control, and is centerable for proper alignment. The condenser is top-mounted for easier replacement when working with additional condensers. The illuminator's field lens provides an iris diaphragm to control light-spread, optimizing contrast and definition in specimen features.

Epi-fluorescence Illumination

AmScope-FM820TA-3MBI3

The epi-fluorescence illumination system adds incredible versatility to the microscope. The ability to isolate fluorescing compounds allows you to target individual proteins and other nuances. This microscope is equipped with a six-position filter turret for easy transitions. Four filter blocks are already mounted (ultraviolet, violet, blue, and green) for popular labeling agents and natural fluorophores. The illuminator uses a high-power mercury arc-lamp for wide-spectrum excitation.



Fluorescence

Filters
Filter SetExcitation FilterDichromatic MirrorEmission Filter
UV-1A330-385nm400nm420nm
V400-410nm455nm455nm
B460-495nm505nm510-550nm
G2510-550nm570nm590nm
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm

Digital Imaging

Monitor and capture photos and videos with the included 3.1MP USB3.0 camera. The highly-sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. A reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

Specifications

Optical SystemInfinite-conjugate
Reference Focal Length200mm
HeadTrinocular, 30° incline, 360° rotatable
Interpupillary AdjustmentSiedentopf compensation-free gemel, 48-75mm
Ocular Diameter30mm
Ocular Dioptersleft ocular
Eyepieces10X, 15X
Photo Portheight-adjustable YX series, 23mm tube mount
Objective LensesInfinity-corrected
Objective Parfocal Distance45mm
Objective Mounting ThreadRMS 20.32mm
Objective TurretReverse quintuple, ball-bearing movement
Focusing SystemCoaxial coarse and fine focus, tension control
Focus Range
Travel24mm
Coarse Stroke37.7mm per rotation
Fine Stroke0.1mm per rotation
Stage DesignDouble-layer with caliper
Stage Dimensions185mm x 142mm
X-Y Translation Range75mm x 55mm
Episcopic Illumination (fluorescence)100W mercury HBO arc-lamp
Fluorescence FiltersUltraviolet: BP330-385nm, DM400nm, BA420nm
Violet: BP400-410nm, DM455nm, BA455nm
Blue: BP460-495nm, DM505nm, BA510-550nm
Green: BP510-550nm, DM570nm, BA590nm
Transmitted Illumination6V30W halogen, continuous-adjustment, rear-mount housing with aspherical collector
CondenserNA0.9/0.25 achromatic swing condenser with iris diaphragm
Sub-stage Condenser-holderrack and pinion

Objective Lenses

MagnificationCorrectionsNAWDImmersion MediumCover-glass Thickness
4Xplan-achromatic0.1028mm
10Xplan-achromatic0.2510mm0.17mm
20Xplan-achromatic0.255.1mm0.17mm
40Xplan-achromatic0.650.7mm0.17mm
100Xplan-achromatic1.250.18mmoil0.17mm

Camera Specifications
Sensor TypeBack-illuminated CMOS
Sensor Optical Format1/2.8"
Active Pixels3.1M (2048 x 1536)
Pixel Size2.5µm x 2.5µm
Active Sensor Area5.12mm x 3.84mm
Shutterelectronic rolling shutter
Sensitivity600mV @ 1/30s
Spectral Response380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate50fps @ 2048x1536
ConnectivityUSB 3.0
Lens-mountC-mount
Power5VDC over USB

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

Packing List:

  • One trinocular head
  • One microscope body
  • One photo port assembly
  • Five objective lenses: 4X, 10X, 20X, 40X, 100X
  • One pair 10X eyepieces
  • One pair 15X eyepieces
  • One stage assembly
  • One swing condenser
  • One halogen illuminator assembly
  • One episcopic illuminator
  • One episcopic lamp housing
  • One power supply for episcopic illuminator
  • One eye shield
  • Two AC power cords (US and Canada standard)
  • One hex driver
  • One dust cover
  • One sample immersion oil
  • One C-mount USB camera
  • One 0.5X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
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