AmScope FM820T-12CMC3 FM820 Series Upright Fluorescence Trinocular Compound Microscope 40X-1000X Magnification with Rotating Multi-filter Turret and 12MP Color CCD Camera

  • Overview
  • Customers Also Bought
  • Your Recently Viewed Items
  • Reviews
AmScope FM820T-12CMC3 FM820 Series Upright Fluorescence Trinocular Compound Microscope 40X-1000X Magnification with Rotating Multi-filter Turret and 12MP Color CCD Camera
Click to zoom
Safe Checkout
Fully secured
Money back Guaranty
100% satisfaction

Overview


AmScope FM820 Series Upright Fluorescence Trinocular Compound Microscope 40X-1000X Magnification with Rotating Multi-filter Turret and 12MP Color CCD Camera

  • An infinity-corrected upright fluorescence microscope with a rotating 6-filter turret
  • Includes 4 excitation filter-blocks: ultraviolet, violet, blue, and green
  • Five magnification levels from 40X to 1000X
  • High-output 100W Mercury-vapor lamp
  • 12MP color CCD camera for low-light fluorescence applications

The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a 40X-1000X magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.

The backwards, quintuple nosepiece holds five plan-achromatic objective lenses, including: 4X, 10X, 20X, 40X, and 100X (oil). The Siedentopf-design head features a 30° incline, and a 48-75mm interpupillary-adjustment range. A trinocular photo-port provides a stable, dedicated mount for an imaging system. The microscope uses coaxial coarse and fine focus-adjustment, as well as coaxial control over X and Y translation of the 7.2" x 5.6" stage.

The 12MP color CCD digital camera and software suite provide a high-resolution imaging solution for fluorescence microscopy. The 1" format CCD sensor balances resolution and sensitivity with its 3.1μm pixels, favoring higher resolution while retaining moderate sensitivity. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Fluorescence

Filters
Filter SetExcitation FilterDichromatic MirrorEmission FilterCommon Stains*
UV-1A330-385nm400nm420nmDAPI, Aniline Blue, BFP, BAO 9, DANSA, Hoechst (33258, 33342, 34580)
V400-410nm455nm455nm
B460-495nm505nm510-550nmAcridine Orange (DNA), Acridine Yellow, Alexa Fluor (430, 488, 500, 514),Cy2, DiO, FITC, Lucifer Yellow
G2510-550nm570nm590nmAcridine Red, Alexa Fluor 532, NeuroTrace 530/615, Rhodamine B
*Common stains are provided as examples, and vary in emission strength. Stain specifications should be verified against filter and light-source specifications.
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. Some fluorophores may emit weak signals despite falling within filter specifications.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm

Specifications

Optical SystemInfinity-corrected
HeadTrinocular, 30 ° incline, 360 ° rotatable
Interpupillary AdjustmentCompensation-free gemel, 48-75mm
EyepiecesPlan 10X, high-eyepoint, FN 22mm, 30mm mount
Photo-port23mm, compatible with reduction lenses
NosepieceQuintuple reversed
Objective Lenses4X, 10X, 20X, 40X (spring), 100X (spring, oil)
Focusing SystemCoaxial coarse and fine focus, upper limit-stop, tension-control
StageDouble-layer mechanical 185mm x 142mm
X-Y Translation Range75mm x 55mm
Sub-stage Condenser-holderRack-and-pinion, centerable
CondenserAchromatic swing condenser, NA0.9/0.25
Transmitted Illumination30W halogen, continuous adjustment
Fluorescence Excitation Illumination100W mercury-vapor HBO arc-lamp

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4XPlan achromatic0.1028mm0.17mm
10XPlan achromatic0.2510mm0.17mm
20XPlan achromatic0.405.1mm0.17mm
40XPlan achromatic0.650.7mm0.17mm
100XPlan achromatic1.250.18mm0.17mm

Camera Specifications

Specifications

Sensor Specifications
ModelSony ICX834AQG (color)
Sensor Typeinterline CCD
Sensor Optical Format1"
Active Pixels12.1M (4284 x 2836)
Pixel Size3.1μm x 3.1μm
Active Sensor Area13.28mm x 8.79mm
Shutterglobal shutter
Sensitivity420mV @ 1/30s (f/5.6)
Spectral Response380-650nm with IR-cut filter
Other Camera Specifications
Binning1x1, 2x2
Video Capture Framerate3.6fps @ 4284x2836
3.6fps @ 2124x1418
Photo Capture Resolution4284x2836
2124x1418
ConnectivityUSB 3.0
Lens-mountC-mount
Compensating Lens1X (optional)
Power5VDC over USB 3.0
Operating Temperature-10-50°C
Operating Humidity30-80%

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons


Packing List:

  • One trinocular head
  • One microscope body
  • One photo port assembly
  • Five objective lenses: 4X, 10X, 20X, 40X, 100X
  • One pair 10X eyepieces
  • One stage assembly
  • One swing condenser
  • One halogen illuminator assembly
  • One episcopic illuminator
  • One episcopic lamp housing
  • One power supply for episcopic illuminator
  • One eye shield
  • Two AC power cords (US and Canada standard)
  • One hex driver
  • One dust cover
  • One sample immersion oil
  • One USB 3.0 CCD Fluorescence Digital Camera
  • One USB 3.0 Cable
  • One C-Mount
⚠️ Attention CA Residents: Prop 65 Warning

Reviews