AmScope IN480TC-FL-3MG3 IN480 Series Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X w/ Kohler Illumination and 2.3MP Global Shutter Camera

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AmScope IN480TC-FL-3MG3 IN480 Series Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X w/ Kohler Illumination and 2.3MP Global Shutter Camera
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  • Brand:AmScope
  • Part #:IN480TC-FL-3MG3
  • Model #:IN480 Series
  • Regular Price: $11,085.99
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Overview


Key Features:

  • 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Includes a highly-sensitive 3.1MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage™s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Filters
Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm

Digital Imaging

The ultra-sensitive 3.1MP camera uses a cutting-edge Sony global-shutter sensor. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Specifications

AmScope-IN480TC-FL-3MG3

Technical drawings with measurements (in mm)


Optical SystemInfinity-corrected
Reference Focal Length220mm
HeadTrinocular, 45° incline
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter30mm
Ocular Dioptersleft ocular
Eyepieces10X, 25X
Photo-portCX-type height-adjustable, 23mm tube
Objective LensesInfinity-corrected
Objective Turretquintuple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
Stagefixed-height, mechanical X-Y translation
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range120mm x 80mm
Specimen HoldersTerasaki plate, Petri dish
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
CondenserNA 0.3, centerable
Condenser Working Distance72mm maximum
Phase Contrast Annuli4X/10X and 20X/40X
Power90-240VAC 50/60Hz wide-band

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm

Camera Specifications
Sensor TypeCMOS (color)
Sensor Optical Format1/1.8"
Active Pixels3.1M (2048 x 1536)
Pixel Size3.45µm x 3.5µm
Active Sensor Area7.07mm x 5.3mm
Shutterglobal shutter
Sensitivity1146mV @ 1/30s
Spectral Response380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate53fps @ 2048x1536
85fps @ 1024x768
ConnectivityUSB 3.0
Lens-mountC-mount
Compensating Lens0.5X
Power5VDC over USB

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One C-mount USB camera
  • One reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
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