AmScope IN480TC-FL-20MBI3 IN480 Series Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X w/ Kohler Illumination and 20MP Back-illuminated CMOS USB 3.0 Camera
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Overview
Key Features:
- 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
- An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
- The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
- The infinity-corrected optical system ensures high-quality, high-resolution images.
- Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
- The trinocular photo port provides a dedicated interface for mounting a camera.
- Monitor and capture photos and videos with the included back-illuminated 20MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.
The Viewing Head
The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.
Optics
The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage™s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.
Koehler Transmitted Illumination
The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.
Epi-fluorescence Illumination
A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.
Fluorescence
Filters | ||||||||||||||||||
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Mercury HBO Excitation | ||||||||||||||||||
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.
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Digital Imaging
Monitor and capture photos and videos with the included high-resolution 20MP USB3.0 camera. The sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.
Specifications
Technical drawings with measurements (in mm)
Optical System | Infinity-corrected | ||||||||
Reference Focal Length | 220mm | ||||||||
Head | Trinocular, 45° incline | ||||||||
Interpupillary Adjustment | Siedentopf compensation-free gemel, 50mm-76mm | ||||||||
Ocular Diameter | 30mm | ||||||||
Ocular Diopters | left ocular | ||||||||
Eyepieces | 10X, 25X | ||||||||
Photo-port | CX-type height-adjustable, 23mm tube | ||||||||
Objective Lenses | Infinity-corrected | ||||||||
Objective Turret | quintuple | ||||||||
Focusing System |
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Stage | fixed-height, mechanical X-Y translation | ||||||||
Stage Dimensions | 160mm x 250mm stage extension: 70mm x 180mm | ||||||||
Stage X-Y Travel Range | 120mm x 80mm | ||||||||
Specimen Holders | Terasaki plate, Petri dish | ||||||||
Episcopic Illumination (Fluorescence) | 100W mercury HBO arc-lamp, centerable | ||||||||
Transmitted Illumination | 6V 30W halogen variable intensity centerable lamp | ||||||||
Condenser | NA 0.3, centerable | ||||||||
Condenser Working Distance | 72mm maximum | ||||||||
Phase Contrast Annuli | 4X/10X and 20X/40X | ||||||||
Power | 90-240VAC 50/60Hz wide-band | ||||||||
Objective Lenses
Magnification | Corrections | NA | Working Distance | Cover-glass Thickness |
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4X | plan achromatic | 0.13 | 10.4mm | 1.5mm |
10X | plan achromatic | 0.25 | 7.30mm | 1.5mm |
20X | plan achromatic | 0.40 | 6.79mm | 1.5mm |
40X | plan achromatic | 0.60 | 3.08mm | 1.5mm |
60X | plan achromatic | 0.70 | 1.71mm | 1.5mm |
10X | phase-ring | 0.25 | 7.30mm | 1.5mm |
20X | phase-ring | 0.40 | 6.80mm | 1.5mm |
40X | phase-ring | 0.60 | 3.08mm | 1.5mm |
Camera Specifications | |
Sensor Type | Back-illuminated CMOS |
Sensor Optical Format | 1" |
Active Pixels | 20M (5440 x 3648) |
Pixel Size | 2.4µm x 2.4µm |
Active Sensor Area | 13.06mm x 8.76mm |
Shutter | electronic rolling shutter |
Sensitivity | 462mV @ 1/30s |
Spectral Response | 380-650nm with IR-cut filter |
Capture Resolution and Maximum Framerate | 15fps @ 5440x3648, 50fps @ 2736x1824, 60fps @ 1824x1216 |
Connectivity | USB 3.0 |
Lens-mount | C-mount |
Compensating Lens | 1X |
Power | 5VDC over USB |
Software
OS Requirements | Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+ | ||||||||||||||||||
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Hardware Requirements | Intel Core2 2.8GHz or comparable processor, 4GB RAM | ||||||||||||||||||
Features |
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