AmScope IN480TC-FL-20MBI3 IN480 Series Inverted Infinity-Corrected Fluorescence Trinocular Compound Microscope 40X-1500X w/ Kohler Illumination and 20MP Back-illuminated CMOS USB 3.0 Camera

Key Features:

• 40X to 1500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for nine unique settings.
• An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
• The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
• The infinity-corrected optical system ensures high-quality, high-resolution images.
• Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
• The trinocular photo port provides a dedicated interface for mounting a camera.
• Monitor and capture photos and videos with the included back-illuminated 20MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.

The IN480T-FL provides a gamut of tools needed by any laboratory performing live cell studies and genetic research. This highly versatile inverted epi-fluorescence microscope is equipped with a Koehler transmitted illumination system for brightfield and phase-contrast observation, and a wide-spectrum episcopic illuminator for fluorescence observation. Two filter blocks are provided for common fluorophores, and additional filters are available for expanded coverage. The large stage and swing-out condenser-holder can accommodate a range of specimen containers including slides, dishes and flasks.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The 23mm photo port is height-adjustable to match focus with the eyepieces, and can be replaced with an optional CX series C-mount port which have built-in reduction, and can directly interface with C-mount cameras.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 25X eyepieces expand the maximum magnification to 1500X with 9 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage™s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Koehler Transmitted Illumination

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Epi-fluorescence Illumination

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

Fluorescence

Digital Imaging

Monitor and capture photos and videos with the included high-resolution 20MP USB3.0 camera. The sensitive back-illuminated sensor is designed for improved low-light imaging, making it suitable for fluorescence and darkfield microscopy. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

Specifications

Technical drawings with measurements (in mm)

Optical System	Infinity-corrected
Reference Focal Length	220mm
Head	Trinocular, 45° incline
Interpupillary Adjustment	Siedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter	30mm
Ocular Diopters	left ocular
Eyepieces	10X, 25X
Photo-port	CX-type height-adjustable, 23mm tube
Objective Lenses	Infinity-corrected
Objective Turret	quintuple
Focusing System	Focus Adjustment Coaxial Coarse/Fine, adjustable tension Fine Division 0.002mm Coarse Stroke 38mm Fine Stroke 0.2mm
Stage	fixed-height, mechanical X-Y translation
Stage Dimensions	160mm x 250mm stage extension: 70mm x 180mm
Stage X-Y Travel Range	120mm x 80mm
Specimen Holders	Terasaki plate, Petri dish
Episcopic Illumination (Fluorescence)	100W mercury HBO arc-lamp, centerable
Transmitted Illumination	6V 30W halogen variable intensity centerable lamp
Condenser	NA 0.3, centerable
Condenser Working Distance	72mm maximum
Phase Contrast Annuli	4X/10X and 20X/40X
Power	90-240VAC 50/60Hz wide-band

Objective Lenses

Magnification	Corrections	NA	Working Distance	Cover-glass Thickness
4X	plan achromatic	0.13	10.4mm	1.5mm
10X	plan achromatic	0.25	7.30mm	1.5mm
20X	plan achromatic	0.40	6.79mm	1.5mm
40X	plan achromatic	0.60	3.08mm	1.5mm
60X	plan achromatic	0.70	1.71mm	1.5mm
10X	phase-ring	0.25	7.30mm	1.5mm
20X	phase-ring	0.40	6.80mm	1.5mm
40X	phase-ring	0.60	3.08mm	1.5mm

Camera Specifications
Sensor Type	Back-illuminated CMOS
Sensor Optical Format	1"
Active Pixels	20M (5440 x 3648)
Pixel Size	2.4µm x 2.4µm
Active Sensor Area	13.06mm x 8.76mm
Shutter	electronic rolling shutter
Sensitivity	462mV @ 1/30s
Spectral Response	380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate	15fps @ 5440x3648, 50fps @ 2736x1824, 60fps @ 1824x1216
Connectivity	USB 3.0
Lens-mount	C-mount
Compensating Lens	1X
Power	5VDC over USB

Software

OS Requirements	Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware Requirements	Intel Core2 2.8GHz or comparable processor, 4GB RAM
Features	Windows Version Live video preview Captures still images and video Time-lapse capture Exposure and white-balance controls Dark-field correction Digital binning Image processing Measurement tools, including lines, arcs, polygons Segmentation and counting tools Image-stitching, extended depth-of-field, HDR Fluorescence color-mapping Mac/Linux Versions Live video preview Captures still images and video Exposure control Color adjustment Measurement tools, including lines, arcs, polygons

Packing List: