AmScope IN400TB-3M3 IN400 Series Inverted Infinity-Corrected Brightfield and Phase-Contrast Biological Trinocular Compound Microscope 40X-1200X w/ 30W Halogen, Quintuple Head and 3MP USB 3.0 C-mount Camera
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Overview
Key Features:
- 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
- A relatively compact inverted microscope with brightfield and phase-contrast for transmitted-light applications.
- The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
- The fixed-height overhead illuminator can be quickly switched from brightfield to phase-contrast using the 3-position annulus board.
- The infinity-corrected optical system ensures high-quality, high-resolution images.
- Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications.
- The trinocular photo port provides a dedicated interface for mounting a camera.
- Monitor and capture photos and videos with the included 3MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.
The IN400T provides high-resolution optics and an inverted illumination system in a stable, ergonomic design for observing cell cultures and other biological and chemical specimens in brightfield and phase-contrast. The inverted form accommodates larger specimen containers, with multiple specimen templates and mechanical stage controls to improve workflow. High-resolution, infinity-corrected optics and phase-contrast capability maximize contrast and clarity to ensure high quality images.
The Viewing Head
The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The photo port is designed to work with 23mm reduction lenses for optimized field-of-view, and is height-adjustable to match focus with the eyepieces.
Optics
The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage's aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.
Transmitted Illumination
The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.
Phase Contrast
The 10X, 20X and 40X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the 3-position phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.
Digital Imaging
Monitor and capture photos and videos with the included 3.0MP USB3.0 camera. The C-mount camera is specially designed for use with microscopes, allowing you to watch live images, and to record photos or videos on your computer. This ability to view microscopic images on your computer reduces eye-strain, and allows groups of people to view images at the same time. The USB 3.0 connection provides greater bandwidth for faster frame-rates. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.
Specifications
Technical drawings with measurements (in mm)
Optical System | Infinity-corrected | ||||||||
Reference Focal Length | 220mm | ||||||||
Head | Trinocular, 45° incline | ||||||||
Interpupillary Adjustment | Siedentopf compensation-free gemel, 50mm-76mm | ||||||||
Ocular Diameter | 30mm | ||||||||
Ocular Diopters | left ocular | ||||||||
Eyepieces | 10X, 20X | ||||||||
Photo-port | CX-type height-adjustable, 23mm tube | ||||||||
Objective Lenses | Infinity-corrected | ||||||||
Objective Turret | quintuple | ||||||||
Focusing System |
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Stage | fixed-height, mechanical X-Y translation | ||||||||
Stage Dimensions | 160mm x 250mm stage extension: 70mm x 180mm | ||||||||
Stage X-Y Travel Range | 120mm x 80mm | ||||||||
Specimen Holders | Terasaki plate, Petri dish | ||||||||
Transmitted Illumination | 6V 30W halogen variable intensity centerable lamp | ||||||||
Condenser | NA 0.3, fixed-position | ||||||||
Condenser Working Distance | 72mm maximum | ||||||||
Phase Contrast Annuli | 4X/10X and 20X/40X | ||||||||
Power | 90-240VAC 50/60Hz wide-band | ||||||||
Objective Lenses
Magnification | Corrections | NA | Working Distance | Cover-glass Thickness |
---|---|---|---|---|
4X | plan achromatic | 0.13 | 10.4mm | 1.5mm |
10X | plan achromatic | 0.25 | 7.30mm | 1.5mm |
20X | plan achromatic | 0.40 | 6.79mm | 1.5mm |
40X | plan achromatic | 0.60 | 3.08mm | 1.5mm |
60X | plan achromatic | 0.70 | 1.71mm | 1.5mm |
10X | phase-ring | 0.25 | 7.30mm | 1.5mm |
20X | phase-ring | 0.40 | 6.80mm | 1.5mm |
40X | phase-ring | 0.60 | 3.08mm | 1.5mm |
Camera Specifications | |
Sensor Type | CMOS |
Sensor Optical Format | 1/3" |
Active Pixels | 3M (2048 x 1534) |
Pixel Size | 2.2µm x 2.2µm |
Active Sensor Area | 4.51mm x 3.37mm |
Shutter | electronic rolling shutter |
Sensitivity | 1.9V/Lux-sec |
380-650nm with IR-cut filter | |
Capture Resolution and Maximum Framerate | 28fps @ 2048x1534 53fps @ 1024x770 |
Connectivity | USB 3.0 |
Lens-mount | C-mount |
Compensating Lens | 0.37X |
Power | 5VDC over USB |
Software
OS Requirements | Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+ | ||||||||||||||||||
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Hardware Requirements | Intel Core2 2.8GHz or comparable processor, 4GB RAM | ||||||||||||||||||
Features |
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