AmScope IN400TB-3M3 IN400 Series Inverted Infinity-Corrected Brightfield and Phase-Contrast Biological Trinocular Compound Microscope 40X-1200X w/ 30W Halogen, Quintuple Head and 3MP USB 3.0 C-mount Camera

Key Features:

• 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
• A relatively compact inverted microscope with brightfield and phase-contrast for transmitted-light applications.
• The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
• The fixed-height overhead illuminator can be quickly switched from brightfield to phase-contrast using the 3-position annulus board.
• The infinity-corrected optical system ensures high-quality, high-resolution images.
• Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications.
• The trinocular photo port provides a dedicated interface for mounting a camera.
• Monitor and capture photos and videos with the included 3MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.

The IN400T provides high-resolution optics and an inverted illumination system in a stable, ergonomic design for observing cell cultures and other biological and chemical specimens in brightfield and phase-contrast. The inverted form accommodates larger specimen containers, with multiple specimen templates and mechanical stage controls to improve workflow. High-resolution, infinity-corrected optics and phase-contrast capability maximize contrast and clarity to ensure high quality images.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The photo port is designed to work with 23mm reduction lenses for optimized field-of-view, and is height-adjustable to match focus with the eyepieces.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage's aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Transmitted Illumination

The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Phase Contrast

The 10X, 20X and 40X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the 3-position phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

Digital Imaging

Monitor and capture photos and videos with the included 3.0MP USB3.0 camera. The C-mount camera is specially designed for use with microscopes, allowing you to watch live images, and to record photos or videos on your computer. This ability to view microscopic images on your computer reduces eye-strain, and allows groups of people to view images at the same time. The USB 3.0 connection provides greater bandwidth for faster frame-rates. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

Specifications

Technical drawings with measurements (in mm)

Optical System	Infinity-corrected
Reference Focal Length	220mm
Head	Trinocular, 45° incline
Interpupillary Adjustment	Siedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter	30mm
Ocular Diopters	left ocular
Eyepieces	10X, 20X
Photo-port	CX-type height-adjustable, 23mm tube
Objective Lenses	Infinity-corrected
Objective Turret	quintuple
Focusing System	Focus Adjustment Coaxial Coarse/Fine, adjustable tension Fine Division 0.002mm Coarse Stroke 38mm Fine Stroke 0.2mm
Stage	fixed-height, mechanical X-Y translation
Stage Dimensions	160mm x 250mm stage extension: 70mm x 180mm
Stage X-Y Travel Range	120mm x 80mm
Specimen Holders	Terasaki plate, Petri dish
Transmitted Illumination	6V 30W halogen variable intensity centerable lamp
Condenser	NA 0.3, fixed-position
Condenser Working Distance	72mm maximum
Phase Contrast Annuli	4X/10X and 20X/40X
Power	90-240VAC 50/60Hz wide-band

Objective Lenses

Magnification	Corrections	NA	Working Distance	Cover-glass Thickness
4X	plan achromatic	0.13	10.4mm	1.5mm
10X	plan achromatic	0.25	7.30mm	1.5mm
20X	plan achromatic	0.40	6.79mm	1.5mm
40X	plan achromatic	0.60	3.08mm	1.5mm
60X	plan achromatic	0.70	1.71mm	1.5mm
10X	phase-ring	0.25	7.30mm	1.5mm
20X	phase-ring	0.40	6.80mm	1.5mm
40X	phase-ring	0.60	3.08mm	1.5mm

Spectral Response

Camera Specifications
Sensor Type	CMOS
Sensor Optical Format	1/3"
Active Pixels	3M (2048 x 1534)
Pixel Size	2.2µm x 2.2µm
Active Sensor Area	4.51mm x 3.37mm
Shutter	electronic rolling shutter
Sensitivity	1.9V/Lux-sec
380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate	28fps @ 2048x1534 53fps @ 1024x770
Connectivity	USB 3.0
Lens-mount	C-mount
Compensating Lens	0.37X
Power	5VDC over USB

Software

OS Requirements	Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware Requirements	Intel Core2 2.8GHz or comparable processor, 4GB RAM
Features	Windows Version Live video preview Captures still images and video Time-lapse capture Exposure and white-balance controls Dark-field correction Digital binning Image processing Measurement tools, including lines, arcs, polygons Segmentation and counting tools Image-stitching, extended depth-of-field, HDR Fluorescence color-mapping Mac/Linux Versions Live video preview Captures still images and video Exposure control Color adjustment Measurement tools, including lines, arcs, polygons

Packing List: