AmScope IN400TB-3M3 IN400 Series Inverted Infinity-Corrected Brightfield and Phase-Contrast Biological Trinocular Compound Microscope 40X-1200X w/ 30W Halogen, Quintuple Head and 3MP USB 3.0 C-mount Camera

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AmScope IN400TB-3M3 IN400 Series Inverted Infinity-Corrected Brightfield and Phase-Contrast Biological Trinocular Compound Microscope 40X-1200X w/ 30W Halogen, Quintuple Head and 3MP USB 3.0 C-mount Camera
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  • Brand:AmScope
  • Part #:IN400TB-3M3
  • Model #:IN400 Series
  • Regular Price: $5,573.99
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Overview


Key Features:

  • 40X to 1200X expanded magnification range using a reversed quintuple lens turret and additional 20X eyepieces for eight unique settings.
  • A relatively compact inverted microscope with brightfield and phase-contrast for transmitted-light applications.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The fixed-height overhead illuminator can be quickly switched from brightfield to phase-contrast using the 3-position annulus board.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included 3MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.

The IN400T provides high-resolution optics and an inverted illumination system in a stable, ergonomic design for observing cell cultures and other biological and chemical specimens in brightfield and phase-contrast. The inverted form accommodates larger specimen containers, with multiple specimen templates and mechanical stage controls to improve workflow. High-resolution, infinity-corrected optics and phase-contrast capability maximize contrast and clarity to ensure high quality images.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging. The photo port is designed to work with 23mm reduction lenses for optimized field-of-view, and is height-adjustable to match focus with the eyepieces.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 600X. Additional 20X eyepieces expand the maximum magnification to 1200X with 8 unique levels. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks thanks to a number of included stage templates. Two plates are supplied for the stage's aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. An extension is provided for an additional 70mm width when needed.

Transmitted Illumination

The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Phase Contrast

The 10X, 20X and 40X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the 3-position phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

Digital Imaging

Monitor and capture photos and videos with the included 3.0MP USB3.0 camera. The C-mount camera is specially designed for use with microscopes, allowing you to watch live images, and to record photos or videos on your computer. This ability to view microscopic images on your computer reduces eye-strain, and allows groups of people to view images at the same time. The USB 3.0 connection provides greater bandwidth for faster frame-rates. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

Specifications

AmScope-IN400TB-3M3

Technical drawings with measurements (in mm)


Optical SystemInfinity-corrected
Reference Focal Length220mm
HeadTrinocular, 45° incline
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter30mm
Ocular Dioptersleft ocular
Eyepieces10X, 20X
Photo-portCX-type height-adjustable, 23mm tube
Objective LensesInfinity-corrected
Objective Turretquintuple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
Stagefixed-height, mechanical X-Y translation
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range120mm x 80mm
Specimen HoldersTerasaki plate, Petri dish
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
CondenserNA 0.3, fixed-position
Condenser Working Distance72mm maximum
Phase Contrast Annuli4X/10X and 20X/40X
Power90-240VAC 50/60Hz wide-band

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm

Spectral Response
Camera Specifications
Sensor TypeCMOS
Sensor Optical Format1/3"
Active Pixels3M (2048 x 1534)
Pixel Size2.2µm x 2.2µm
Active Sensor Area4.51mm x 3.37mm
Shutterelectronic rolling shutter
Sensitivity1.9V/Lux-sec
380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate28fps @ 2048x1534
53fps @ 1024x770
ConnectivityUSB 3.0
Lens-mountC-mount
Compensating Lens0.37X
Power5VDC over USB

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

Packing List:

  • One microscope
  • One photo port
  • One mechanical stage attachment
  • One illuminator
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair 10X eyepieces
  • One pair 20X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • One bulb
  • One power cable
  • One dust cover
  • One C-mount USB camera
  • One 0.37X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to type A cable
  • One software CD
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