IN300TC-FL-MF IN300 Series Inverted Fluorescence Trinocular Compound Microscope 40X-1000X Magnification with Phase-contrast and 3MP SCMOS Camera by AmScope
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Overview
Features
- Designed for fluorescence microscopy, this microscope uses a high-power arc-lamp to cover a wide excitation range.
- The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
- Four plan-fluor objective lenses and two sets of eyepieces provide seven unique magnifications ranging from 40X to 1000X
- Specialized 10X and 20X objective lenses are included for phase-contrast applications.
- Includes a 3MP SCMOS camera and specialized microscopy software for PC
The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design.
The microscope uses an infinity-corrected optical system with an expanded magnificatin range of 40X to 1000X. The four plan-fluor objectives lenses provide high-resolution, color-corrected images with edge-to-edge sharpness. The 10X and 20X lenses can be used in conjunction with the provided phase-annulus plate for phase-contrast.
For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.
The inverted format provides a platform for a wide range of vessels, including slides, culture dishes and flasks, and Terasaki plates. A camera can be easily mounted to the dedicated photo port for a live video feed and digital imaging. A wide range of cameras is available, including CCD and back-illuminated CMOS models designed specifically for low-light applications.
The 3MP color sCMOS camera is designed for low-light applications. It captures still images and video, and allows you to view a live stream on your PC. The included Windows software offers image-development and measurement tools. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images.
Fluorescence
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| Mercury HBO Excitation | |||||||||||||||
| Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.
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Specifications
| Optical System | Infinity-corrected | ||||
| Head | Trinocular, 45° incline | ||||
| Interpupillary Adjustment | Siedentopf compensation-free gemel, 50mm-75mm | ||||
| Ocular Diameter | 30mm | ||||
| Eyepieces | 10X FN22mm plan wide-field, 25X wide-field | ||||
| Photo-port | 23mm tube, height-adjustable | ||||
| Objective Turret | quadruple | ||||
| Focusing System |
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| Stage | fixed-height, mechanical X-Y translation | ||||
| Stage Dimensions | 172mm x 242mm | ||||
| Stage X-Y Travel Range | 75mm x 40mm | ||||
| Specimen Holders | Terasaki plate, Petri dish | ||||
| Transmitted Illumination | 12V 30W halogen variable intensity centerable lamp | ||||
| Condenser | NA 0.3, iris diaphragm, filter slot | ||||
| Power | 110VAC 60Hz | ||||
Objective Lenses
| Magnification | Corrections | NA | Working Distance | Cover-glass Thickness |
|---|---|---|---|---|
| 4X | Plan fluor | 0.15 | 28mm | 1.5mm |
| 10X | Plan fluor, phase-contrast | 0.30 | 3mm | 1.5mm |
| 20X | Plan fluor, phase-contrast | 0.50 | 4.27mm | 1.5mm |
| 40X | Plan fluor | 0.60 | 2.23mm | 1.5mm |
Magnification Chart
| Eyepiece | Objective | |||
| 4X | 10X | 20X | 40X | |
| 10X | 40X | 100X | 200X | 400X |
| 25X | 100X | 250X | 500X | 1000X |
Camera Specifications
| Sensor Type | sCMOS (color) |
| Sensor Optical Format | 1/2.8" |
| Active Pixels | 3.1M (2048 x 1536) |
| Pixel Size | 2.5µm x 2.5µm |
| Active Sensor Area | 5.12mm x 3.84mm |
| Shutter | electronic rolling shutter |
| Connectivity | USB 2.0 |
| Lens-mount | C-mount |
| Power | 5V over USB |
Software
| OS Requirements | Windows (32/64 bit) XP/Vista/7/8/10 | ||||||
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| Features |
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