AmScope IN300T-FL-MBI3 IN300 Series Inverted Phase-Contrast Biological EPI-Fluorescent Trinocular Compound Microscope 40X-400X w/ 30W Halogen and 1.2MP USB 3.0 C-mount Camera

  • Overview
  • Customers Also Bought
  • Your Recently Viewed Items
  • Reviews
AmScope IN300T-FL-MBI3 IN300 Series Inverted Phase-Contrast Biological EPI-Fluorescent Trinocular Compound Microscope 40X-400X w/ 30W Halogen and 1.2MP USB 3.0 C-mount Camera
Click to zoom
  • Brand:AmScope
  • Part #:IN300T-FL-MBI3
  • Model #:IN300 Series
Safe Checkout
Fully secured
Money back Guaranty
100% satisfaction

Overview


Key Features:

  • 40X to 400X magnification range using a quadruple lens turret for four unique settings.
  • An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • The infinity-corrected optical system ensures high-quality, high-resolution images.
  • Specialized 10X and 20X objective lenses are included for phase-contrast applications.
  • The trinocular photo port provides a dedicated interface for mounting a camera.
  • Monitor and capture photos and videos with the included ultra-sensitive 1.2MP USB 3.0 camera. Professional microscopy software for Windows, Mac, and Linux provide tools for image processing, measuring, and more.

The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity.

The Viewing Head

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces.

Optics

The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness.

The Stage

The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage's aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement.

Transmitted Illumination

The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

Phase Contrast

The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light.

Epi-fluorescence Illumination

For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available.

Fluorescence

Filters
Filter SetExcitation FilterEmission Filter
B450-490nm520nm
G510-560nm590nm
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm

Digital Imaging

Monitor and capture photos and videos with the included 1.2MP USB3.0 camera. With extraordinary sensitivity and high framerates, the MU123-FL provides high-definition imaging for low-light applications, including fluorescence and darkfield. Using a cutting-edge 1.2MP back-illuminated CMOS sensor with an incredible 2040mV sensitivity (1/30s), high gain-levels can be used for highly-responsive realtime video. A 0.37X reduction lens is included for use with 23mm-30.5mm ports to capture more of the microscope's field-of-view. Our professional software for Windows provides a wide assortment of capture and photo-editing functions including color correction, time-lapse capture, image stitching, and a full complement of measuring tools. A lite version of our software is available for Mac and Linux with essential functionality for capturing photos and videos.

Specifications

Optical SystemInfinity-corrected
HeadTrinocular, 45° incline
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-75mm
Ocular Diameter30mm
Ocular Dioptersright ocular
Eyepieces10X
Photo-portheight-adjustable 23mm tube
Objective LensesInfinity-corrected
Objective TurretQuadruple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Stagefixed-height, mechanical X-Y translation
Stage Dimensions172mm x 242mm
Stage X-Y Travel Range75mm x 40mm
Specimen HoldersTerasaki plate, Petri dish
Episcopic Illumination (Fluorescence)100W mercury HBO arc-lamp, centerable
Transmitted Illumination12V 30W halogen
variable intensity
CondenserNA 0.3, iris diaphragm, filter slot
Power110VAC 60Hz

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan fluor0.1528mm1.5mm
10Xplan fluor, phase-contrast0.303mm1.5mm
20Xplan fluor, phase-contrast0.504.27mm1.5mm
40Xplan fluor0.602.23mm1.5mm

Camera Specifications
Sensor TypeBack-illuminated CMOS
Sensor Optical Format1/3"
Active Pixels1.2M (1280 x 960)
Pixel Size3.75µm x 3.75µm
Active Sensor Area4.80mm x 3.60mm
Shutterelectronic rolling shutter
Sensitivity2040mV @ 1/30s
Spectral Response380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate60fps @ 1280x960
120fps @ 640x480
ConnectivityUSB 3.0
Lens-mountC-mount
Compensating Lens0.37X
Power5VDC over USB

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

Packing List:

  • One microscope body
  • Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X
  • One pair 10X eyepieces
  • One phase-contrast annulus board
  • One top-mount illuminator
  • One rear-mount fluorescence illuminator
  • One power supply for fluorescence illuminator
  • One UV blocking shield
  • One 30W halogen bulb
  • One 100W mercury bulb
  • One dust cover
  • Color filters
  • Two power cords
  • One C-mount USB camera
  • One 0.37X reduction lens
  • Two sizing adapters
  • One USB 3.0 Type B to USB 3.0 type A cable
  • One software CD
  • Reviews