Frequently Asked Questions
What are the main types of chromatography columns?
HPLC (reverse-phase/normal-phase), GC, ion-exchange, size-exclusion (SEC), and affinity chromatography columns. Each separates by different interactions – polarity, charge, size, or specific binding.
How do I choose the right HPLC column?
Match column to analyte and method: start with C18 for non-polars; try phenyl/cyano/amide for selectivity. Pick particle size and dimensions for resolution vs backpressure. Check pH/solvent limits before you buy chromatography equipment.
How long does a chromatography column last and how can I extend its life?
From dozens to hundreds of runs. To extend: filter samples, use a guard column, keep within pH/pressure limits, flush after buffers, store in a compatible solvent, and avoid particulates – habits that protect chromatography columns.