AmScope IN480TC-FL-BWF IN480 Series Inverted Infinity-corrected Phase-contrast Trinocular Compound Microscope 40X-1500X Magnification with Koehler Illumination and 1.4MP Monochrome CCD Camera

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AmScope IN480TC-FL-BWF IN480 Series Inverted Infinity-corrected Phase-contrast Trinocular Compound Microscope 40X-1500X Magnification with Koehler Illumination and 1.4MP Monochrome CCD Camera
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Overview


AmScope IN480 Series Inverted Infinity-corrected Phase-contrast Trinocular Compound Microscope 40X-1500X Magnification with Koehler Illumination and 1.4MP Monochrome CCD Camera

  • Designed for fluorescence microscopy, this microscope uses a high-power arc-lamp to cover a wide excitation range.
  • The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers.
  • Five plan-achromatic objective lenses and two sets of eyepieces provide nine unique magnifications ranging from 40X to 1500X
  • The infinity-corrected optical system eliminates certain optical errors when using filters and other in-line components, ensuring highly-precise images.
  • Specialized 10X, 20X, and 40X objective lenses are included for phase-contrast applications, further improved by the fully-customizable Koehler illumination system.
  • Includes a highly-sensitive 1.4MP monochrome CCD USB 2.0 camera with active cooling, and specialized microscopy software for PC

The IN480T-FL provides high-resolution optics and a versatile dual-illumination system with Koehler transmitted lighting for brightfield and phase-contrast, and wide-spectrum episcopic lighting for fluorescence. The stable and ergonomic inverted form accommodates larger specimen containers, with multiple specimen templates and mechanical stage controls to improve workflow.

The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. A sliding shutter isolates the light path when using a camera, avoiding stray light from the oculars during fluorescence imaging.

The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly-precise images. Five brightfield objective lenses and two sets of eyepieces provide a wide magnification range from 40X to 1500X, with plan-achromatic corrections for high resolution, accurate colors, and edge-to-edge sharpness. Additionally, three phase-ring equipped objective lenses can be used for phase-contrast observation.

A 100W mercury-vapor episcopic illuminator provides wide-spectrum lighting for fluorescence excitation. Two filter blocks can be mounted and alternated during observation. Blue and green excitation blocks are included to cover a wide range of common fluorophores.

The Koehler transmitted illumination system consists of a centerable 30W halogen lamp, a field lens with iris, and a centerable, height-adjustable condenser with iris. The ability to center the lamp and condenser, along with the field and aperture diaphragms, allow for highly-efficient, optimized illumination for maximum resolution. Along with height adjustment, the condenser can be swung out of the light path. A slide-in annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction.

The 1.4MP monochrome CCD camera provides extremely high sensitivity with active Peltier cooling system designed to prevent noise from excess heat. rolled, low-light imaging solution which is ideal for long-exposure fluorescence microscopy. The camera captures still images and video, and allows you to view a live stream on your PC. The interline CCD design uses global-shutter capture which eliminates artifacts associated with rolling-shutter sensors. The included Windows software offers image-development and measurement tools. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images.

This microscope operates on 90-240V wide-band voltage for use in multiple international regions.

Fluorescence

Filters
Filter SetExcitation Center Wavelength / BandwidthDichromatic Mirror Cut-on WavelengthEmission Center Wavelength / BandwidthCommon Stains
B1480nm/30nm505nm535nm/40nmAlexa Fluor 488, Cy2, FITC
G1560nm/40nm600nm635nm/60nmTexas Red, Cy3.5, Mito Tracker Red
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm

Specifications

Optical SystemInfinity-corrected
HeadTrinocular, 45° incline
Interpupillary AdjustmentSiedentopf compensation-free gemel, 50mm-76mm
Ocular Diameter30mm
Eyepieces10X FN22mm plan wide-field, 25X wide-field
Photo-portCX-type height-adjustable, 23mm tube
Objective Turretquintuple
Focusing System
Focus AdjustmentCoaxial Coarse/Fine, adjustable tension
Fine Division0.002mm
Coarse Stroke38mm
Fine Stroke0.2mm
Stagefixed-height, mechanical X-Y translation
Stage Dimensions160mm x 250mm
stage extension: 70mm x 180mm
Stage X-Y Travel Range120mm x 80mm
Specimen HoldersTerasaki plate, Petri dish
Transmitted Illumination6V 30W halogen
variable intensity
centerable lamp
CondenserNA 0.3, centerable
Condenser Working Distance72mm maximum
Phase Contrast Annuli4X/10X and 20X/40X
Power90-240VAC 50/60Hz wide-band

Objective Lenses

MagnificationCorrectionsNAWorking DistanceCover-glass Thickness
4Xplan achromatic0.1310.4mm1.5mm
10Xplan achromatic0.257.30mm1.5mm
20Xplan achromatic0.406.79mm1.5mm
40Xplan achromatic0.603.08mm1.5mm
60Xplan achromatic0.701.71mm1.5mm
10Xphase-ring0.257.30mm1.5mm
20Xphase-ring0.406.80mm1.5mm
40Xphase-ring0.603.08mm1.5mm

Magnification Chart

EyepieceObjective
4X10X20X40X60X
10X40X100X200X400X600X
25X100X250X500X1000X1500X

Camera Specifications

Sensor TypeInterline CCD (monochrome)
Sensor Optical Format2/3"
Active Pixels1.4M (1360 x 1024)
Pixel Size6.45µm x 6.45µm
Active Sensor Area8.77mm x 6.6mm
Shutterglobal shutter
Sensitivity1300mV @ 1/30s
Spectral Response380-650nm with IR-cut filter
Capture Resolution and Maximum Framerate7.5fps @ 1360x1024
15fps @ 680x512
Cooling-30°C below ambient
ConnectivityUSB 3.0
Lens-mountC-mount
PowerEternal 12V, 3A

Software

OS RequirementsWindows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware RequirementsIntel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

Contents

  • One microscope
  • One 23mm photo port
  • One mechanical stage attachment
  • One condenser
  • Five brightfield objective lenses
  • Three phase-contrast objective lenses
  • One pair of 10X eyepieces
  • One pair of 25X eyepieces
  • One glass stage insert
  • One metal stage insert
  • Three specimen holders
  • One phase-contrast annulus board
  • One centering telescope
  • Two bulbs
  • Two power cables
  • One dust cover
  • One fluorescence lamp housing
  • One power supply for fluorescence lamp
  • One mercury HBO bulb
  • One B1 filter block
  • One G1 filter block
  • One UV-blocking light shild
  • One camera
  • One camera power adapter
  • One 0.65X C-mount photo port
  • One USB cable
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