AmScope FM820TC Upright EPI-Fluorescence Trinocular Compound Microscope 40X-2500X Magnification w/ Rotating Multi-Filter Turret
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Overview
AmScope FM820 Series Upright EPI-Fluorescence Trinocular Compound Microscope 40X-2500X Magnification with Rotating Multi-Filter Turret
- 40X to 2500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for eight unique settings.
- A scalable epi-fluorescence microscope for laboratories and universities with wide-spectrum 100W mercury HBO arc-lamp and four turret-mounted filter blocks
- Includes ultraviolet, violet, blue, and green excitation filter blocks for popular fluorochromes
- A robust, high-performance microscope with superior optics and mechanical design
- Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification
- The infinity-corrected optical system reduces distortion and improves color accuracy.
- The Koehler illumination system provides centered, evenly-distributed lighting with a field aperture for maximum contrast and definition.
- The trinocular head provides a dedicated photo port for mounting a camera.
The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a wide magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.
Viewing Head
The microscope's head is designed for precision and comfort. The super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. Compensation-free Siedentopf interpupillary adjustment allows any user to properly align the oculars without readjusting focus. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The photo port is equipped with a 23mm tube for attaching cameras with compensating lenses, and is height-adjustable to match focus with the eyepieces. The port is compatible with our YX series C-mount adapters which can attach directly to C-mount cameras without a compensating lens " ideal for 1 format sensors. The head is set to a 30° incline which is ideal for viewing from a standing position, and it can be rotated 360°.
Optics
The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 1000X. Additional 25X eyepieces expand the maximum magnification to 2500X with 8 unique levels. The turret's reversed orientation provides more working room above the stage. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.
The Stage
The 2-layer mechanical stage provides smooth and precise movement for examination of specimen slides, with a large translation range for viewing larger samples. The stage's low-position controls are conveniently placed near the coaxial focus knobs for a streamlined workflow. Examining specimen slides is intuitive and precise for users at any skill level. Symmetrical focus controls are coaxially mounted for fast, intuitive focusing. A height limit can be set to prevent contact damage, as well as presetting optimal focus distance.
Koehler Transmitted Illumination
Built on Koehler illumination principals, the sub-stage lighting provides evenly-distributed light for optimal contrast. The rear-mounted 30W halogen light-source provides 100 CRI illumination with ample brightness for contrast-enhancement methods like darkfield. The lower color temperature of the halogen light-source causes less eyestrain compared to high-Kelvin LED. The light condenser uses a swing mechanism for optimized lighting at both high and low magnifications, with a NA0.25 setting for full coverage at extremely low magnifications. An iris diaphragm allows you to adjust the aperture to optimize contrast and depth-of-field. The condenser holder uses rack-and-pinion height adjustment for precision control, and is centerable for proper alignment. The condenser is top-mounted for easier replacement when working with additional condensers. The illuminator's field lens provides an iris diaphragm to control light-spread, optimizing contrast and definition in specimen features.
Epi-fluorescence Illumination
The epi-fluorescence illumination system adds incredible versatility to the microscope. The ability to isolate fluorescing compounds allows you to target individual proteins and other nuances. This microscope is equipped with a six-position filter turret for easy transitions. Four filter blocks are already mounted (ultraviolet, violet, blue, and green) for popular labeling agents and natural fluorophores. The illuminator uses a high-power mercury arc-lamp for wide-spectrum excitation.
Fluorescence
Filters | |||||||||||||||||||||||||
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Mercury HBO Excitation | |||||||||||||||||||||||||
Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output.
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Specifications
Optical System | Infinite-conjugate | ||||||
Reference Focal Length | 200mm | ||||||
Head | Trinocular, 30° incline, 360° rotatable | ||||||
Interpupillary Adjustment | Siedentopf compensation-free gemel, 48-75mm | ||||||
Ocular Diameter | 30mm | ||||||
Ocular Diopters | left ocular | ||||||
Eyepieces | 10X, 25X | ||||||
Photo Port | height-adjustable YX series, 23mm tube mount | ||||||
Objective Lenses | Infinity-corrected | ||||||
Objective Parfocal Distance | 45mm | ||||||
Objective Mounting Thread | RMS 20.32mm | ||||||
Objective Turret | Reverse quintuple, ball-bearing movement | ||||||
Focusing System | Coaxial coarse and fine focus, tension control | ||||||
Focus Range |
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Stage Design | Double-layer with caliper | ||||||
Stage Dimensions | 185mm x 142mm | ||||||
X-Y Translation Range | 75mm x 55mm | ||||||
Episcopic Illumination (fluorescence) | 100W mercury HBO arc-lamp | ||||||
Transmitted Illumination | 6V30W halogen, continuous-adjustment, rear-mount housing with aspherical collector | ||||||
Condenser | NA0.9/0.25 achromatic swing condenser with iris diaphragm | ||||||
Sub-stage Condenser-holder | rack and pinion | ||||||
Objective Lenses
Magnification | Corrections | NA | WD | Immersion Medium | Cover-glass Thickness |
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4X | plan-achromatic | 0.10 | 28mm | — | — |
10X | plan-achromatic | 0.25 | 10mm | — | 0.17mm |
20X | plan-achromatic | 0.25 | 5.1mm | — | 0.17mm |
40X | plan-achromatic | 0.65 | 0.7mm | — | 0.17mm |
100X | plan-achromatic | 1.25 | 0.18mm | oil | 0.17mm |