AmScope FM820TC Upright EPI-Fluorescence Trinocular Compound Microscope 40X-2500X Magnification w/ Rotating Multi-Filter Turret

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AmScope FM820TC Upright EPI-Fluorescence Trinocular Compound Microscope 40X-2500X Magnification w/ Rotating Multi-Filter Turret
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  • Brand:AmScope
  • Part #:FM820TC
  • Model #:FM820 Series
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Overview


AmScope FM820 Series Upright EPI-Fluorescence Trinocular Compound Microscope 40X-2500X Magnification with Rotating Multi-Filter Turret

  • 40X to 2500X expanded magnification range using a reversed quintuple lens turret and additional 25X eyepieces for eight unique settings.
  • A scalable epi-fluorescence microscope for laboratories and universities with wide-spectrum 100W mercury HBO arc-lamp and four turret-mounted filter blocks
  • Includes ultraviolet, violet, blue, and green excitation filter blocks for popular fluorochromes
  • A robust, high-performance microscope with superior optics and mechanical design
  • Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification
  • The infinity-corrected optical system reduces distortion and improves color accuracy.
  • The Koehler illumination system provides centered, evenly-distributed lighting with a field aperture for maximum contrast and definition.
  • The trinocular head provides a dedicated photo port for mounting a camera.

The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a wide magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.

Viewing Head

The microscope's head is designed for precision and comfort. The super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. Compensation-free Siedentopf interpupillary adjustment allows any user to properly align the oculars without readjusting focus. For users with astigmatism or asymmetrical vision, the left ocular can be adjusted to fine-tune focus. The photo port is equipped with a 23mm tube for attaching cameras with compensating lenses, and is height-adjustable to match focus with the eyepieces. The port is compatible with our YX series C-mount adapters which can attach directly to C-mount cameras without a compensating lens " ideal for 1 format sensors. The head is set to a 30° incline which is ideal for viewing from a standing position, and it can be rotated 360°.

Optics

AmScope-FM820TC

The infinity-corrected optical system eliminates optical errors when using filters and other in-line components, ensuring highly precise images. The quintuple objective turret provides instant access to 5 magnification levels. This allows you to efficiently move through magnifications from 40X to 1000X. Additional 25X eyepieces expand the maximum magnification to 2500X with 8 unique levels. The turret's reversed orientation provides more working room above the stage. These high-quality lenses are achromatically corrected to improve resolution and color accuracy, with planatic flat-field correction to provide edge-to-edge sharpness.



The Stage

The 2-layer mechanical stage provides smooth and precise movement for examination of specimen slides, with a large translation range for viewing larger samples. The stage's low-position controls are conveniently placed near the coaxial focus knobs for a streamlined workflow. Examining specimen slides is intuitive and precise for users at any skill level. Symmetrical focus controls are coaxially mounted for fast, intuitive focusing. A height limit can be set to prevent contact damage, as well as presetting optimal focus distance.

Koehler Transmitted Illumination

Built on Koehler illumination principals, the sub-stage lighting provides evenly-distributed light for optimal contrast. The rear-mounted 30W halogen light-source provides 100 CRI illumination with ample brightness for contrast-enhancement methods like darkfield. The lower color temperature of the halogen light-source causes less eyestrain compared to high-Kelvin LED. The light condenser uses a swing mechanism for optimized lighting at both high and low magnifications, with a NA0.25 setting for full coverage at extremely low magnifications. An iris diaphragm allows you to adjust the aperture to optimize contrast and depth-of-field. The condenser holder uses rack-and-pinion height adjustment for precision control, and is centerable for proper alignment. The condenser is top-mounted for easier replacement when working with additional condensers. The illuminator's field lens provides an iris diaphragm to control light-spread, optimizing contrast and definition in specimen features.

Epi-fluorescence Illumination

AmScope-FM820TC

The epi-fluorescence illumination system adds incredible versatility to the microscope. The ability to isolate fluorescing compounds allows you to target individual proteins and other nuances. This microscope is equipped with a six-position filter turret for easy transitions. Four filter blocks are already mounted (ultraviolet, violet, blue, and green) for popular labeling agents and natural fluorophores. The illuminator uses a high-power mercury arc-lamp for wide-spectrum excitation.



Fluorescence

Filters
Filter SetExcitation FilterDichromatic MirrorEmission Filter
UV-1A330-385nm400nm420nm
V400-410nm455nm455nm
B460-495nm505nm510-550nm
G2510-550nm570nm590nm
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output.

BandPeak Wavelength
UV334nm, 365nm
V405nm
B436nm
G546nm
Y578nm

Specifications

Optical SystemInfinite-conjugate
Reference Focal Length200mm
HeadTrinocular, 30° incline, 360° rotatable
Interpupillary AdjustmentSiedentopf compensation-free gemel, 48-75mm
Ocular Diameter30mm
Ocular Dioptersleft ocular
Eyepieces10X, 25X
Photo Portheight-adjustable YX series, 23mm tube mount
Objective LensesInfinity-corrected
Objective Parfocal Distance45mm
Objective Mounting ThreadRMS 20.32mm
Objective TurretReverse quintuple, ball-bearing movement
Focusing SystemCoaxial coarse and fine focus, tension control
Focus Range
Travel24mm
Coarse Stroke37.7mm per rotation
Fine Stroke0.1mm per rotation
Stage DesignDouble-layer with caliper
Stage Dimensions185mm x 142mm
X-Y Translation Range75mm x 55mm
Episcopic Illumination (fluorescence)100W mercury HBO arc-lamp
Transmitted Illumination6V30W halogen, continuous-adjustment, rear-mount housing with aspherical collector
CondenserNA0.9/0.25 achromatic swing condenser with iris diaphragm
Sub-stage Condenser-holderrack and pinion

Objective Lenses

MagnificationCorrectionsNAWDImmersion MediumCover-glass Thickness
4Xplan-achromatic0.1028mm
10Xplan-achromatic0.2510mm0.17mm
20Xplan-achromatic0.255.1mm0.17mm
40Xplan-achromatic0.650.7mm0.17mm
100Xplan-achromatic1.250.18mmoil0.17mm

Packing List:

  • One trinocular head
  • One microscope body
  • One photo port assembly
  • Five objective lenses: 4X, 10X, 20X, 40X, 100X
  • One pair 10X eyepieces
  • One pair 25X eyepieces
  • One stage assembly
  • One swing condenser
  • One halogen illuminator assembly
  • One episcopic illuminator
  • One episcopic lamp housing
  • One power supply for episcopic illuminator
  • One eye shield
  • Two AC power cords (US and Canada standard)
  • One hex driver
  • One dust cover
  • One sample immersion oil
  • ⚠️ Attention CA Residents: Prop 65 Warning

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