AmScope FM820T-9CMC3 FM820 Series Upright Fluorescence Trinocular Compound Microscope 40X-1000X Magnification with Rotating Multi-filter Turret and 9MP Color CCD Camera

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AmScope FM820T-9CMC3 FM820 Series Upright Fluorescence Trinocular Compound Microscope 40X-1000X Magnification with Rotating Multi-filter Turret and 9MP Color CCD Camera
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Overview

Features

  • An infinity-corrected upright fluorescence microscope with a rotating 6-filter turret
  • Includes 4 excitation filter-blocks: ultraviolet, violet, blue, and green
  • Five magnification levels from 40X to 1000X
  • High-output 100W Mercury-vapor lamp
  • 9MP color CCD camera for low-light fluorescence applications

The FM820T is an upright fluorescence microscope with transmitted and reflected lighting, and a six-filter turret. The microscope is built around an infinity-corrected optical system with a 40X-1000X magnification range, and Koehler diascopic and episcopic illumination. The powerful 100W, wide-spectrum, mercury-vapor episcopic illuminator uses a rotary filter-selector which can house up to six fluorescence filter-blocks; already included are ultraviolet, violet, blue, and green excitation blocks. The sub-stage illuminator uses a 30W halogen lamp with field-lens and diaphragm for even, high-contrast lighting.

The backwards, quintuple nosepiece holds five plan-achromatic objective lenses, including: 4X, 10X, 20X, 40X, and 100X (oil). The Siedentopf-design head features a 30° incline, and a 48-75mm interpupillary-adjustment range. A trinocular photo-port provides a stable, dedicated mount for an imaging system. The microscope uses coaxial coarse and fine focus-adjustment, as well as coaxial control over X and Y translation of the 7.2" x 5.6" stage.

The 9MP color CCD digital camera and software suite provide a high-resolution imaging solution for fluorescence microscopy. The 1" format CCD sensor balances resolution and sensitivity with its 3.69µm pixels, favoring higher resolution while retaining moderate sensitivity. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it's a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Fluorescence

Filters
Filter Set Excitation Filter Dichromatic Mirror Emission Filter Common Stains*
UV-1A 330-385nm 400nm 420nm DAPI, Aniline Blue, BFP, BAO 9, DANSA, Hoechst (33258, 33342, 34580)
V 400-410nm 455nm 455nm
B 460-495nm 505nm 510-550nm Acridine Orange (DNA), Acridine Yellow, Alexa Fluor (430, 488, 500, 514),Cy2, DiO, FITC, Lucifer Yellow
G2 510-550nm 570nm 590nm Acridine Red, Alexa Fluor 532, NeuroTrace 530/615, Rhodamine B
*Common stains are provided as examples, and vary in emission strength. Stain specifications should be verified against filter and light-source specifications.
Mercury HBO Excitation

Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. Some fluorophores may emit weak signals despite falling within filter specifications.

Band Peak Wavelength
UV 334nm, 365nm
V 405nm
B 436nm
G 546nm
Y 578nm

Specifications

Optical System Infinity-corrected
Head Trinocular, 30 ° incline, 360 ° rotatable
Interpupillary Adjustment Compensation-free gemel, 48-75mm
Eyepieces Plan 10X, high-eyepoint, FN 22mm, 30mm mount
Photo-port 23mm, compatible with reduction lenses
Nosepiece Quintuple reversed
Objective Lenses 4X, 10X, 20X, 40X (spring), 100X (spring, oil)
Focusing System Coaxial coarse and fine focus, upper limit-stop, tension-control
Stage Double-layer mechanical 185mm x 142mm
X-Y Translation Range 75mm x 55mm
Sub-stage Condenser-holder Rack-and-pinion, centerable
Condenser Achromatic swing condenser, NA0.9/0.25
Transmitted Illumination 30W halogen, continuous adjustment
Fluorescence Excitation Illumination 100W mercury-vapor HBO arc-lamp

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X Plan achromatic 0.10 28mm 0.17mm
10X Plan achromatic 0.25 10mm 0.17mm
20X Plan achromatic 0.40 5.1mm 0.17mm
40X Plan achromatic 0.65 0.7mm 0.17mm
100X Plan achromatic 1.25 0.18mm 0.17mm

Camera Specifications

Specifications

Sensor Specifications
Model Sony ICX814AQG (color)
Sensor Type interline CCD
Sensor Optical Format 1"
Active Pixels 9M (3388 x 2712)
Pixel Size 3.69µm x 3.69µm
Active Sensor Area 12.5mm x 10.01mm
Shutter global shutter
Sensitivity 580mV @ 1/30s (f/5.6)
Spectral Response 380-650nm with IR-cut filter
Other Camera Specifications
Binning 1x1, 2x2
Video Capture Framerate 4.4fps @ 3388x2712
4.4fps @ 1694x1356
Photo Capture Resolution 3388x2712
1694x1356
Connectivity USB 3.0
Lens-mount C-mount
Compensating Lens 1X (optional)
Power 5VDC over USB 3.0
Operating Temperature -10-50°C
Operating Humidity 30-80%

Software

OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+
Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM
Features
Windows Version
Live video preview
Captures still images and video
Time-lapse capture
Exposure and white-balance controls
Dark-field correction
Digital binning
Image processing
Measurement tools, including lines, arcs, polygons
Segmentation and counting tools
Image-stitching, extended depth-of-field, HDR
Fluorescence color-mapping
Mac/Linux Versions
Live video preview
Captures still images and video
Exposure control
Color adjustment
Measurement tools, including lines, arcs, polygons

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